负载TGF-β3壳聚糖复合水凝胶促进兔半月板缺损修复

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (11): 1512-1518.

负载TGF-β3壳聚糖复合水凝胶促进兔半月板缺损修复

王赟¹, 徐宏宇², 潘宗友³, 倪利承¹, 王刚祥², 赵勇², 郑强^1*

1.浙江大学医学院附属第二医院骨科,浙江杭州 300009;
2.嵊州市人民医院骨科,浙江嵊州 312400;
3.浙江大学医学院附属第一医院骨科,浙江杭州 310003

收稿日期:2019-12-20 修回日期:2020-07-24 出版日期:2020-11-05 发布日期:2020-10-30
通讯作者: ^* 2100057@zju.edu.cn

Chitosan composite hydrogel loaded with TGF-β3 promotes the repair of meniscus defects in rabbits

WANG Yun¹, XU Hong-yu², PAN Zong-you³, NI Li-cheng¹, WANG Gang-xiang², ZHAO Yong², ZHENG Qiang^1*

1. Department of Orthopaedics, the Second Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 300009;
2. Department of Orthopaedics, Shengzhou People's Hospital, Shengzhou 312400,China;
3. Department of Orthopaedics, the First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003

Received:2019-12-20 Revised:2020-07-24 Online:2020-11-05 Published:2020-10-30
Contact: ^* 2100057@zju.edu.cn

摘要/Abstract

摘要： 目的研究骨髓间充质干细胞(BM-MSCs)与负载转化生长因子β3(TGF-β3)的壳聚糖复合水凝胶的复合物修复半月板缺损的效果。方法利用60只新西兰大白兔制作半月板前角全层缺损模型。将模型兔随机分为:对照组(未植入任何材料)、修复Ⅰ组(缺损处注入壳聚糖复合水凝胶与骨髓间充质干细胞复合物)、修复Ⅱ组(缺损处注入负载TGF-β3的壳聚糖复合水凝胶与骨髓间充质干细胞复合物)。术后12周取材,进行放射学检查、大体和组织学观察;利用Western blot检测Ⅱ型胶原、蛋白聚糖蛋白水平。结果对照组的半月板前角全层缺损,无明显修复迹象;修复Ⅰ组的缺损部位大部分被修复组织填充;修复Ⅱ组的缺损部位完全被修复组织填充。修复Ⅱ组的Ⅱ型胶原、蛋白聚糖蛋白表达高于对照组(P＜0.05)。结论骨髓间充质干细胞与负载TGF-β3的壳聚糖水凝胶联合可促进兔半月板缺损的修复。

关键词: 壳聚糖复合水凝胶, 转化生长因子β3, 半月板缺损, Ⅱ型胶原, 蛋白聚糖

Abstract: Objective To study the effect of bone marrow mesenchymal stem cells(BM-MSCs) combined with chitosan composite hydrogel loaded with transforming growth factor beta 3 (TGF-β3) to repair meniscus defects. Methods A full-thickness defect model of the anterior horn of the meniscus was developed in 60 New Zealand white rabbits. They were randomly divided into: the control group which did not implant any materials. The repair group Ⅰ in which chitosan composite hydrogel and bone marrow mesenchymal stem cells composite were injected into the defect group, and in group Ⅱ,the chitosan composite hydrogel loaded with TGF-beta 3 and bone marrow mesenchymal stem cells complex were repaired. After 12 weeks of operation, the samples were taken for radiology, gross and histological observation. Western blot was used to detect type Ⅱ collagen and proteoglycan protein. Results In the control group, the full-thickness defect of the meniscus anterior horn was not obviously repaired; in the repair group Ⅰ,most of the defects were filled with repaired tissue; in the repair group Ⅱ, the defects were completely filled with repaired tissue. The expression level of type Ⅱ collagen and proteoglycan protein in repair group Ⅱ was higher than that in control group (P＜0.05). Conclusions Bone marrow mesenchymal stem cells combined with chitosan hydrogel loaded with TGF-beta 3 can promote the repair of meniscus defects in rabbits.

Key words: chitosan composite hydrogel, transforming growth factor beta 3, meniscus defect, type Ⅱ collagen, proteoglycan

中图分类号:

R683

王赟, 徐宏宇, 潘宗友, 倪利承, 王刚祥, 赵勇, 郑强. 负载TGF-β3壳聚糖复合水凝胶促进兔半月板缺损修复[J]. 基础医学与临床, 2020, 40(11): 1512-1518.

WANG Yun, XU Hong-yu, PAN Zong-you, NI Li-cheng, WANG Gang-xiang, ZHAO Yong, ZHENG Qiang. Chitosan composite hydrogel loaded with TGF-β3 promotes the repair of meniscus defects in rabbits[J]. Basic & Clinical Medicine, 2020, 40(11): 1512-1518.

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