抑制miR-621表达促进人胃癌细胞系增殖、迁移和侵袭

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (11): 1500-1505.

抑制miR-621表达促进人胃癌细胞系增殖、迁移和侵袭

陈国雁, 冯晓波^*

上海中医药大学附属第七人民医院消化内科, 上海 200137

收稿日期:2019-08-26 修回日期:2020-02-08 出版日期:2020-11-05 发布日期:2020-10-30
通讯作者: ^* yaojo08430@163.com

Inhibition of miR-621 expression promotes proliferation, migration and invasion of human gastric cancer cell lines

CHEN Guo-yan, FENG Xiao-bo^*

Department of Gastroenterology, the Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China

Received:2019-08-26 Revised:2020-02-08 Online:2020-11-05 Published:2020-10-30
Contact: ^* yaojo08430@163.com

摘要/Abstract

摘要： 目的检测miR-621在胃癌组织及胃癌细胞系中的表达情况,评估miR-621的预后价值,分析miR-621对胃癌细胞生物学功能的影响。方法荧光定量PCR检测癌和癌旁组织以及胃癌细胞系中miR-621的表达。用卡方检验分析miR-621与胃癌患者临床病理学特征之间的关系;用生存曲线和Cox回归分析miR-621对胃癌的预后价值。最后转染胃癌细胞miR-621的模拟物和抑制剂,通过CCK-8法、Transwell小室法分析胃癌细胞增殖、迁移和侵袭能力。结果胃癌组织以及胃癌细胞系中miR-621的表达量显著低于对照组(P＜0.01),且与胃癌患者的淋巴结转移、肿瘤组织分化和TNM分期显著相关(P＜0.05)。同时发现miR-621可以作为胃癌患者的独立预后因子(HR=3.09, 95% CI=1.31~7.27, P=0.01),且miR-621表达水平低的患者生存率较差(log rank test P＜0.001)。此外,抑制miR-621的表达水平能显著的增加胃癌细胞的增殖、迁移和侵袭的能力(P＜0.05)。结论 miR-621的低表达与胃癌的不良预后相关,并且会促进胃癌细胞的增殖、迁移和侵袭能力。

关键词: 胃癌, miR-621, 预后, 生物学功能

Abstract: Objective To detect the expression of miR-621 in gastric cancer tissues and cell lines and to evaluate the prognostic significance of miR-621, and to analyze the effect of miR-621 on the biological function of gastric cancer cells. Methods Expression of miR-621 in cancer tissue and cell lines was detected by real-time polymorperase chain reaction(RT-qPCR). Chi-square test was used to analyze the relationship between miR-621 and clinical pathological features of gastric cancer patients. Prognostic significance of miR-621 for gastric cancer was analyzed by survival curve and Cox regression. Finally, human gastric cancer cells were transfected with miR-621 mimics and inhibitors to analyze the proliferation, migration and invasion ability of gastric cancer cells. Results The expression of miR-621 was significantly downregulated in gastric cancer tissues and cell lines as compared to the adjacent tissues and gastric mucosa cell lines(P＜0.01), and low expression was associated with lymph node metastasis, tumor differentiation, and TNM stage in gastric cancer(P＜0.05). The patients with low expression of miR-621 had a poor overall survival rates(log-rank test P＜0.001), and miR-621 was an independent prognostic indicator for gastric cancer patients(HR=3.09, 95% CI=1.31-7.27, P=0.01). In addition, low-expression of miR-621 significantly increased proliferation, migration, and invasion of gastric cancer cells(P＜0.05). Conclusions Low expression of miR-621 is associated with poor prognosis of gastric cancer, which promotes the proliferation, migration and invasion of gastric cancer cells.

Key words: gastric cancer, miR-621, prognosis, biological function

中图分类号:

R73-3

陈国雁, 冯晓波. 抑制miR-621表达促进人胃癌细胞系增殖、迁移和侵袭[J]. 基础医学与临床, 2020, 40(11): 1500-1505.

CHEN Guo-yan, FENG Xiao-bo. Inhibition of miR-621 expression promotes proliferation, migration and invasion of human gastric cancer cell lines[J]. Basic & Clinical Medicine, 2020, 40(11): 1500-1505.

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