TCF7L2 rs290481基因多态性与2型糖尿病合并冠心病相关

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (11): 1484-1488.

TCF7L2 rs290481基因多态性与2型糖尿病合并冠心病相关

骆时木, 欧阳航, 蒋燕成, 张志珊^*

福建医科大学附属泉州第一医院检验科, 福建泉州 362000

收稿日期:2020-03-24 修回日期:2020-07-01 出版日期:2020-11-05 发布日期:2020-10-30
通讯作者: ^* 554882707@qq.com
基金资助:
泉州市高层次人才创新创业项目(2017Z038)

TCF7L2 rs290481 polymorphisms are correlated to type 2 diabetes combined with coronary heart disease

LUO Shi-mu, OUYANG Hang, JIANG Yan-cheng, ZHANG Zhi-shan^*

Department of Clinical Laboratory, Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou 362000, China

Received:2020-03-24 Revised:2020-07-01 Online:2020-11-05 Published:2020-10-30
Contact: ^* 554882707@qq.com

摘要/Abstract

摘要： 目的探讨转录因子7类似物(TCF7L2)基因rs290481位点单核苷酸多态性(SNPs)与2型糖尿病合并冠心病的相关性。方法选取泉州地区汉族120例单纯2型糖尿病(T2DM)患者、120例冠心病(CHD)患者、120例2型糖尿病伴发冠心病(T2DM+CHD)患者和120名健康对照者(control)。提取外周血DNA,用聚合酶链式反应(PCR)扩增TCF7L2后测序;用χ²检验法分析不同组别间基因型分布频率的差异;用两分类Logistic回归分析糖尿病合并冠心病的危险因素。结果 T2DM组TCF7L2基因rs290481 AA、AG、GG基因型分布频率与对照组、CHD组、T2DM+CHD组比较均有明显差异(P＜ 0.01)。AA基因型和AG/GG基因型之间HbAlc、GLU水平有明显差异(P＜0.05)。Logistic回归表明,高血压病史、HbAlc、GlU和TCF7L2基因rs290481位点AG/GG基因型是T2DM合并CHD发生的独立危险因素。结论 TCF7L2 rs290481基因位点多态性与T2MD合并CHD相关,AG/GG基因型较AA基因型患者T2MD合并CHD的患病风险较高。

关键词: 单核苷酸多态性, 聚合酶链式反应, 等位基因, 突变

Abstract: Objective To investigate the correlation between single nucleotide polymorphisms (SNPs) on the rs290481 site of TCF7L2 gene and type 2 diabetes mellitus complicated with coronary heart disease(CHD). Methods Peripheral blood DNA was extracted and TCF7L2 gene was amplified by polymerase chain reaction (PCR) and then sequenced in 120 patients with simple type 2 diabetes (T2DM), 120 patients with coronary heart disease (CHD), 120 patients with type 2 diabetes combined with coronary (T2DM+CHD) and 120 healthy controls. Chi-square test was used to analyze the difference of genotype frequency in four groups and binary logistic regression was used to analyze the risk factors of diabetes with coronary heart disease. Results The distribution frequency of AA, AG and GG on TCF7L2 and rs290481 SNP sites from T2DM group were significantly different with the controls, CHD group and T2DM plus CHD group respectively (P＜0.01). The serum level of HbAlc and GLU, was significantly different between AA and AG/GG genotypes (P＜0.05). Logistic regression data demonstrated that history of hypertension, HbAlc, GLU and the rs290481 AG/GG genotype of TCF7L2 were independent risk factors of diabetes mellitus complicated with CHD. Conclusions The polymorphism of TCF7L2 rs290481 gene is associated with the risk of type 2 diabetes complicated with CHD. AG/GG genotype confer a significantly increased risk of type 2 diabetes mellitus with CHD.

Key words: single nucleotide polymorphism, polymerase chain reaction, allele, mutation

中图分类号:

骆时木, 欧阳航, 蒋燕成, 张志珊. TCF7L2 rs290481基因多态性与2型糖尿病合并冠心病相关[J]. 基础医学与临床, 2020, 40(11): 1484-1488.

LUO Shi-mu, OUYANG Hang, JIANG Yan-cheng, ZHANG Zhi-shan. TCF7L2 rs290481 polymorphisms are correlated to type 2 diabetes combined with coronary heart disease[J]. Basic & Clinical Medicine, 2020, 40(11): 1484-1488.

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