人尿液细胞来源的诱导多能干细胞定向分化为毛囊干细胞

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (11): 1468-1473.

人尿液细胞来源的诱导多能干细胞定向分化为毛囊干细胞

周化腾^*, 李长明, 王利祥, 胡劲涛, 杜伟斌, 章何陋, 任伟凡

浙江中医药大学附属江南医院骨科,浙江杭州 311201

收稿日期:2020-05-17 修回日期:2020-07-27 出版日期:2020-11-05 发布日期:2020-10-30
通讯作者: ^* zeallo@foxmail.com
基金资助:
国家自然科学基金青年科学基金(81904053);浙江省医药卫生科技计划(2018KY654);杭州市萧山区科技计划(201709)

Differentiation of human urine-derived iPSCs into hair follicle stem cells

ZHOU Hua-teng^*, LI Chang-ming, WANG Li-xiang, HU Jin-tao, DU Wei-bin, ZHANG He-lou, REN Wei-fan

Department of Orthopedics, Affiliated Jiangnan Hospital of Zhejiang Chinese Medical University, Hangzhou 311201, China

Received:2020-05-17 Revised:2020-07-27 Online:2020-11-05 Published:2020-10-30
Contact: ^* zeallo@foxmail.com

摘要/Abstract

摘要： 目的观察诱导多能干细胞(iPSCs)定向分化为毛囊干细胞(HFSCs)的潜能。方法通过4个转录因子(OCT4、SOX2、KLF4和c-Myc)重编程健康人尿液细胞为诱导多能干细胞(iPSCs)。通过调控维甲酸(RA)、骨形态发生蛋白4(BMP-4)、表皮细胞生长因子(EGF)信号通路,将iPSCs诱导为毛囊干细胞(HFSCs),并对iPSCs来源的毛囊干细胞(iPS-HFSCs)进行免疫细胞化学分析、RT-qPCR。结果人尿液细胞来源的iPSCs具有胚胎干细胞(ESCs)类似的细胞形态、基因表达。通过调控RA、BMP-4及EGF信号通路,iPSCs定向分化为具有类似毛囊干细胞形态、基因表达和表型的细胞。结论通过4个转录因子,尿液细胞成功重编程为全能性的iPSCs。iPSCs具有毛囊干细胞类似特性,为构建基于iPSCs的组织工程皮肤提供参考。

关键词: EGF, 诱导多能干细胞, 毛囊干细胞

Abstract: Objective To differentiate urine derived pluripotent stem cells (iPSCs) into hair follicle stem cells (HFSCs) to construct a skin substitutes. Methods Human urinary cells isolated from a healthy donor were re-programmed into iPSCs. iPSCs were then induced into iPS-HFSCs. Immunocytochemistry and RT-qPCR were used to examine the characteristics of the induced iPS-HFSCs. Results The iPSCs induced from urine cells have characteristics as embryonic stem cells. iPSCs derived cells displayed the morphology, genes expression and phenotypes similar to iPS-HFSCs. Conclusions The urine cells are successfully reprogramed into iPSCs by four transcription factors, and iPSCs derived cells show characteristics similar to HFSCs.

Key words: EGF, induced pluripotent stem cells, hair follicle stem cells

中图分类号:

Q813

周化腾, 李长明, 王利祥, 胡劲涛, 杜伟斌, 章何陋, 任伟凡. 人尿液细胞来源的诱导多能干细胞定向分化为毛囊干细胞[J]. 基础医学与临床, 2020, 40(11): 1468-1473.

ZHOU Hua-teng, LI Chang-ming, WANG Li-xiang, HU Jin-tao, DU Wei-bin, ZHANG He-lou, REN Wei-fan. Differentiation of human urine-derived iPSCs into hair follicle stem cells[J]. Basic & Clinical Medicine, 2020, 40(11): 1468-1473.

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