食管腺癌细胞系及模型和3D培养的研究进展

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (10): 1409-1415.

食管腺癌细胞系及模型和3D培养的研究进展

刘董剑^1,3, 杨凌^2,3*

1.内蒙古医科大学研究生院,内蒙古呼和浩特 010110;
2.内蒙古医科大学附属医院临床医学研究中心,内蒙古呼和浩特 010050;
3.内蒙古自治区医学细胞生物学重点实验室,内蒙古呼和浩特 010050

收稿日期:2020-04-10 修回日期:2020-06-27 出版日期:2020-10-05 发布日期:2020-09-29
通讯作者: ^* yanglingshmily@126.com
基金资助:
国家自然科学基金(31960153); 内蒙古自治区自然科学基金(2018MS08076)

Advances in the study of esophageal adenocarcinoma cell lines, model develpoment and 3D culture technology

LIU Dong-jian^1,3, YANG Ling^2,3*

1. Graduate School of Inner Mongolia Medical University, Hohhot 010110;
2. Clinical Medicine Research Center, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050;
3. Inner Mongolia Key Laboratory of Medical Cell Biology, Hohhot 010050, China

Received:2020-04-10 Revised:2020-06-27 Online:2020-10-05 Published:2020-09-29
Contact: ^* yanglingshmily@126.com

摘要/Abstract

摘要： 食管腺癌是一种少见的恶性肿瘤。食管腺癌恶化和转移的性质为肿瘤的治疗带来了一定程度的困难和挑战。近年来,食管腺癌细胞系研究取得了一定程度的进展,但肿瘤细胞内微观分子水平的发生机制还有许多未被知晓,食管腺癌细胞间的差异性仍需要继续探索。食管腺癌细胞模型的建立和发展可用于研究肿瘤细胞性质和特点,各种肿瘤细胞模型为食管腺癌的发生机制提供了研究工具,有利于了解肿瘤细胞内基因的异常表达和微观信号分子对肿瘤增殖的影响因素。类器官的3D-培养能够为肿瘤细胞的异性质和微观水平表达的研究提供理想的平台,为推进对肿瘤细胞性质的研究提供了有利的条件。

关键词: 食管腺癌, 异种移植物, 3D培养, 类器官

Abstract: Esophageal adenocarcinoma is a uncommon malignant tumor. The deterioration and metastasis of esophageal adenocarcinoma bring some difficulties and challenges to the treatment of tumor. In recent years, some progress has been made in the study of esophageal adenocarcinoma cell lines. However, there are still many unknown molecular mechanisms in tumor cells, and the differences between esophageal adenocarcinoma cells still need to be further explored. The establishment and development of esophageal adenocarcinoma cell model can be used to study the properties and characteristics of tumor cells, and various tumor cell models provide research tools for the mechanism of esophageal adenocarcinoma pathogenesis, which is conducive to understanding the abnormal expression of genes in tumor cells and the influence factors of microsignaling molecules on tumor proliferation. The 3D-culture of organoid can provide an ideal platform for the study of the heterogeneous properties and microscopic level expression of tumor cells, and provide favorable conditions for the advancement of the study of the properties of tumor cells.

Key words: esophageal adenocarcinoma, xenograft, 3D-culture, organoid

中图分类号:

R735.1

刘董剑, 杨凌. 食管腺癌细胞系及模型和3D培养的研究进展[J]. 基础医学与临床, 2020, 40(10): 1409-1415.

LIU Dong-jian, YANG Ling. Advances in the study of esophageal adenocarcinoma cell lines, model develpoment and 3D culture technology[J]. Basic & Clinical Medicine, 2020, 40(10): 1409-1415.

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