己糖激酶2促进人宫颈癌细胞系的迁移与增殖

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (10): 1320-1327.

己糖激酶2促进人宫颈癌细胞系的迁移与增殖

雷丹¹, 张燕茹¹, 陈茜¹, 李露^2*, 崔南^1*

1.西安交通大学第一附属医院生殖医学科,西安交通大学医学部,陕西西安710061;
2.西安医学院第一附属医院肾病科,陕西西安 710021

收稿日期:2020-05-20 修回日期:2020-07-27 出版日期:2020-10-05 发布日期:2020-09-29
通讯作者: ^* cuin2003@xjtufh.edu.cn; liluwenqi@163.com
基金资助:
国家自然科学基金(81903042)

HK2 promotes migration and proliferation of human cervical cancer cell lines

LEI Dan¹, ZHANG Yan-ru¹, CHEN Qian¹, LI Lu^2*, CUI Nan^1*

1. Department of Reproductive Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Medical College of Xi'an Jiaotong University, Xi'an 710061;
2. Department of Nephrology, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710021, China

Received:2020-05-20 Revised:2020-07-27 Online:2020-10-05 Published:2020-09-29
Contact: ^* cuin2003@xjtufh.edu.cn; liluwenqi@163.com

摘要/Abstract

摘要： 目的研究己糖激酶2(HK2)通过p-ERK1/2促进人子宫颈癌细胞HeLa增殖和迁移的分子机制。方法用Western blot和细胞免疫化学检测HK2在宫颈癌细胞系中的表达;用G418压力筛选获取HK2稳定表达的HeLa-HK2及对照细胞系;用细胞划痕和Transwell小室法检测HeLa-HK2及对照细胞的迁移和侵袭;用细胞计数、MTT法、流式细胞仪检测HeLa-HK2及对照细胞的增殖、细胞活力及细胞周期的分布;用Western blot和细胞免疫化学检测ERK1/2、p-ERK1/2、cyclin A和Slug蛋白的表达;用ERK抑制剂(FR180204)抑制ERK1/2和p-ERK1/2表达后观察HeLa-HK2细胞增殖、迁移和侵袭能力的变化。结果成功构建HK2稳定表达的HeLa-HK2细胞系;HeLa-HK2细胞迁移、侵袭及增殖能力显著强于对照细胞,HeLa-HK2细胞中p-ERK1/2、cyclin A和Slug蛋白表达高于对照细胞;使用FR180204在HeLa-HK2细胞中抑制ERK1/2和p-ERK1/2表达后,可以抑制HK2对细胞增殖、迁移和侵袭的增强作用。结论 HK2通过p-ERK1/2上调cyclin A和Slug蛋白表达促进细胞的增殖、迁移和侵袭。

关键词: 己糖激酶2, p-ERK1/2, Slug, cyclin A, 宫颈癌

Abstract: Objective To investigate the molecular mechanism of HK2 on promoting cell proliferation and migration through p-ERK1/2 in cervical cancer cell line HeLa. Methods Expression of HK2 in cervical cancer cell lines was detected by Western blot and immunocychemistry. Over-expressed HK2 cell line(HeLa-HK2) was established by using G418. Wound-healing and Transwell cells assays were used to detect the migratory and invasive potential in HeLa-HK2 and in control cell lines. Cell growth curves, MTT assay and flow cytometry were used to detect cell proliferation, cell viability and cell cycle. The expression of ERK1/2, p-ERK1/2, cyclin A and Slug was detected by using Western blot and immunocytochemistry. The ERK inhibitor (FR180204) was used to inhibit expression of ERK1/2 and p-ERK1/2, and the potential of cell proliferation, migration and invasion was observed in these cells. Results HeLa-HK2 cells that stably expressing HK2 was successfully constructed. HK2 enhanced cell migration, invasion and growth potential in HeLa cells. The expression of ERK1/2, p-ERK1/2, cyclin A and Slug was higher in HeLa-HK2 cells than those in control cells. The protein level of ERK1/2, p-ERK1/2, cyclin A and Slug was decreased in FR180204 treated HeLa-HK2 cells, and the potential of growth, migratory and invasive were also attenuated by FR180204 in HeLa-HK2 cells. Conclusions HK2 promotes cell growth, migration and invasion in HeLa cells by up-regulating expression of cyclin A and Slug through stimulating p-ERK1/2.

Key words: hexokinase 2, p-ERK1/2, Slug, cyclin A, cervical cancer

中图分类号:

R737.33

雷丹, 张燕茹, 陈茜, 李露, 崔南. 己糖激酶2促进人宫颈癌细胞系的迁移与增殖[J]. 基础医学与临床, 2020, 40(10): 1320-1327.

LEI Dan, ZHANG Yan-ru, CHEN Qian, LI Lu, CUI Nan. HK2 promotes migration and proliferation of human cervical cancer cell lines[J]. Basic & Clinical Medicine, 2020, 40(10): 1320-1327.

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