基础医学与临床 ›› 2019, Vol. 39 ›› Issue (6): 816-820.

• 研究论文 • 上一篇    下一篇

miR-24在胆管癌组织和细胞系HCCC-9810中的表达

窦雯雯,孙广洪,杨敏,谢瑞柱,苏艳,徐建华,赵雪   

  1. 潍坊医学院附属医院
  • 收稿日期:2018-10-25 修回日期:2019-01-24 出版日期:2019-06-05 发布日期:2019-06-04
  • 通讯作者: 孙广洪 E-mail:wo108986chao@163.com

Expression of miR-24 in cholangiocarcinoma tissues and cell line HCCC-9810

  • Received:2018-10-25 Revised:2019-01-24 Online:2019-06-05 Published:2019-06-04

摘要: 目的 探讨microRNA-24(miR-24)在胆管癌患者组织中的表达情况,并分析其表达预后价值及生物学功能。方法 收集124例胆管癌患者的癌组织和癌旁组织标本,用实时荧光定量PCR检测miR-24含量,使用χ2检验对miR-24和胆管癌临床病理学特征的关系进行分析,并通过Kaplan-Meier生存曲线和Cox回归模型评估miR-24在胆管癌中的预后价值。以人胆管癌细胞系HCCC-9810为研究对象,通过转染pEGFP-C1-miR-24融合表达载体,分析miR-24对胆管癌细胞增殖、迁移和侵袭能力的影响。结果 miR-24在胆管癌组织中的表达较癌旁组织上调(P<0.05),且与患者淋巴结转移、肿瘤细胞分化及TNM分期显著相关。miR-24高表达的胆管癌患者的整体生存率显著低于低表达患者(log-rank<0.001),是胆管癌的独立预后因子(HR=5.518, 95%CI=2.394-12.717, P<0.001)。此外,胆管癌细胞中上调的miR-24能够显著地促进癌细胞的增殖、迁移和侵袭的能力。结论 胆管癌中表达水平上调的miR-24与患者不良预后显著相关,并参与了胆管癌的进展过程,可能成为胆管癌治疗的新靶点。

关键词: MiR-24, 胆管癌, 预后, 增殖, 转移

Abstract: Objective To explore the expression patterns of microRNA-24 (miR-24) in cholangiocarcinoma (CCA) tissues, and analyze its prognostic value and biological function in HCC. Methods Total of 124 pairs of CCA tissues and adjacent tissues were collected, and the expression of miR-24 in the tissues was estimated by quantitative real-time RT-PCR (RT-qPCR). χ2 test was used to assess the association of miR-24 with clinicopathological characteristics of the patients. The prognostic value of miR-24 was evaluated by Kaplan-Meier survival curves and Cox regression model. The effect of miR-24 on cell proliferation, migration and invasion was investigated in CCA cell line HCCC-9810 by cell transfection with fusion expression vector pEGFP-C1-miR-24. Results The expression of miR-24 was significantly upregulated in CCA tissues compared with the paired noncancerous tissues (P<0.05), and was associated with lymph node metastasis, differentiation and TNM stage of the patients. The patients with high miR-24 expression had poor overall survival compared with those with low miR-24 expression (log-rank P<0.001). The expression of miR-24 was considered an independent prognostic factor (HR=5.518, 95%CI=2.394-12.717, P<0.001). In addition, the overexpression of miR-24 in CCA cells led to enhanced cell proliferation, migration and invasion. Conclusion The upregulated expression of miR-24 is associated with poor prognosis of CCA, and is involved in tumor progression, which may be a novel target for CCA treatment.

Key words: MiR-24, Cholangiocarcinoma, Prognosis, Proliferation, Migration