基础医学与临床 ›› 2019, Vol. 39 ›› Issue (2): 157-164.

• 研究论文 •    下一篇

新疆哈萨克族人群23个常染色体STR基因座的遗传多态性及与其他民族的遗传相关性

刘亚举1,李效阳2,郭利红2,李学博3,石美森4   

  1. 1. 河南省许昌市公安局刑事科学技术研究所
    2. 河南省公安厅刑事科学技术研究所
    3. 山东政法学院
    4. 中国政法大学 证据科学教育部重点实验室
  • 收稿日期:2018-02-13 修回日期:2018-05-30 出版日期:2019-02-05 发布日期:2019-01-16
  • 通讯作者: 刘亚举 E-mail:horse3697@126.com
  • 基金资助:
    国家自然科学基金

Genetic polymorphisms and genetic relationship of 23 autosomal STR loci in Kazakh population of Xinjiang

  • Received:2018-02-13 Revised:2018-05-30 Online:2019-02-05 Published:2019-01-16

摘要: 目的 调查新疆哈萨克族人群23个常染色体STR基因座遗传多态性,探讨其群体遗传关系及法医学应用价值。方法 用华夏TM白金试剂盒,对新疆地区550名哈萨克族无关个体DNA进行扩增,3500XL遗传分析仪电泳分析,GeneMapper ID-X v1.4软件分析等位基因片段大小。统计分析23个STR基因座的频率数据和法医遗传学参数,并与其他地区已有人群数据进行比较。结果 新疆哈萨克族各基因座个体识别率DP值在0.8206~ 0.9879,多态信息含量 PIC值在0.5888~ 0.9200。累积个体识别率(CDP)和累积非父排除率(CPE)分别为1-9.2942×10-29和1-6.9503×10-11。根据Nei's DA遗传距离计算,本研究的新疆哈萨克族与数据库中的新疆和田哈萨克族的遗传距离最近(0.0044),与西藏藏族的遗传距离相对最远(0.0333)。结论 这23个STR基因座在新疆哈萨克族人群中具有丰富的遗传多态性。研究不同民族群体的遗传多样性对了解他们的起源、迁移以及相互关系有重要的意义。

关键词: 常染色体短串联重复序列, 新疆哈萨克族, Neighbor-Joining系统发育树, 遗传关系

Abstract: Objective To investigate the genetic polymorphisms of 23 autosomal short tandem repeats(STR)loci in Xinjiang Kazakh population, and explore the population genetic relationships and evaluate its application value on forensic medicine. Methods The DNA of 550 unrelated individuals in Xinjiang Kazakh population were amplified using HuaxiaTM Platinum kit, and the PCR products were analyzed by electrophoresis through 3500XL genetic analyzer. The fragment sizes of alleles were analyzed subsequently by GeneMapper ID-X v1.4. Allele frequencies and forensic genetic parameters of 23 STR loci were statistically analyzed and compared with the available data of other populations from different races and regions. Results The power of discrimination(DP), and polymorphic information content(PIC) ranged from 0.8206 to 0.9879, 0.5888 to 0.9200. The total discrimination power and cumulate probability of exclusion were 1-9.2942×10-29 and 1-6.9503×10-11, respectively. Comparing with 23 reference populations, the genetic distance (Nei’s DA) between Xinjiang Kazakh in this study and Xinjiang Hotan Kazakh in database was the smallest(0.0044), while it was relatively larger between Xinjiang Kazakh and Tibet Tibetan (0.0333). Conclusions The results show that the 23 STR provided highly polymorphic information and forensic efficiency for forensic individual identification and paternity testing. The study of genetic diversity among different populations is useful in research of their origins, migrations and their relationships.

Key words: autosomal short tandem repeats, Xinjiang Kazakh, Neighbor-Joining tree, Genetic Relationships