水飞蓟素抑制肝癌细胞系MHCC97侵袭及迁移

›› 2019, Vol. 39 ›› Issue (12): 1741-1745.

水飞蓟素抑制肝癌细胞系MHCC97侵袭及迁移

张健,马海,杨柳

南充市中心医院

收稿日期:2018-12-18 修回日期:2019-05-17 出版日期:2019-12-05 发布日期:2019-12-04
通讯作者: 杨柳 E-mail:635181603@qq.com

Silymarin inhibits invasion and migration of hepatoma cell line MHCC97

Received:2018-12-18 Revised:2019-05-17 Online:2019-12-05 Published:2019-12-04

摘要/Abstract

摘要： 目的探讨水飞蓟素（SM）对人肝癌细胞系系MHCC97侵袭和转移能力的影响及可能涉及的分子机制。方法MTT法检测细胞增殖；Transwell、划痕实验和细胞-基质黏附实验分别检测细胞侵袭，迁移及黏附能力；RT-PCR和Western blot检测MMP-9、VEGF、E-cadherin、integrin β1和Akt mRNA及蛋白的表达。结果和对照组相比，水飞蓟素抑制细胞增殖(P<0.05)，显著降低细胞侵袭，迁移和黏附能力 (P<0.05)。水飞蓟素处理组MMP-9、VEGF、integrin β1和Akt的mRNA及蛋白表达量降低(P<0.05)，而E-cadherin 的mRNA及蛋白表达量增高(P<0.05)。结论水飞蓟素可抑制MHCC97细胞增殖，并且降低细胞侵袭，迁移和黏附能力。

关键词: 肝癌, MHCC97细胞, 水飞蓟素, 侵袭, 迁移

Abstract: Objective To study the effects of silymarin on invasion and migration of MHCC97 cell lines, and explore the possible molecule mechanism. Methods Cell suppression rates were detected by MTT assay. Migration, invasion and adhesion of MHCC97 cells after silymarin treatment were observed by cell migration assay, wound healing assay and cell-matrix adhesion assay. The mRNA and protein levels of MMP9, VEGF, E-cadherin, integrin β1 and Akt were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western Blot separately. Results The silymarin suppressed growth rate of MHCC97 cells; and migration, invasion and adhesion abilities. The mRNA and protein levels of MMP-9, VEGF, Integrin β1 and p-Akt were also reduced, but the expressions of mRNA and protein of E-cadherin increased. Conclusions Silymarin can inhibit the growth of MHCC97 cells, and reduce the invasion, migration, and adhesion abilities of MHCC97 cell lines.

Key words: liver cancer, MHCC97 cells, silymarin, invasion, migration

中图分类号:

张健马海杨柳. 水飞蓟素抑制肝癌细胞系MHCC97侵袭及迁移[J]. , 2019, 39(12): 1741-1745.

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