基础医学与临床 ›› 2019, Vol. 39 ›› Issue (10): 1449-1454.

• 研究论文 • 上一篇    下一篇

PAPD4基因缺失影响小鼠的运动功能

蒋娅玲1,钟古华2,李振超2,王莉2,陈振健2,吴金庆2   

  1. 1. 广东省江门市第三人民医院
    2. 江门市第三人民医院
  • 收稿日期:2018-09-27 修回日期:2019-03-06 出版日期:2019-10-05 发布日期:2019-09-25
  • 通讯作者: 蒋娅玲 E-mail:2427537152@qq.com
  • 基金资助:
    江门市医疗卫生领域科技计划项目

Knockout of PAPD4 gene affects locomotion ability of mouse

  • Received:2018-09-27 Revised:2019-03-06 Online:2019-10-05 Published:2019-09-25

摘要: 目的 研究PAPD4基因敲除对小鼠行为和生理状态的影响。方法 按性别、基因型将小鼠分为雄性对照、雄性全敲、雌性对照和雌性全敲。开放场、水迷宫、血糖检测、血压检测、T迷宫、糖水偏好和强迫游泳。结果 雌性敲除鼠在开放场中的运动速度(p<0.05)和总运动距离(p<0.001)显著少于雌性对照。雌、雄敲除鼠在水迷宫中的运动速度低于同性对照,且雌性小鼠在第六次和第九次训练的运动速度存在显著低于对照(p6<0.05, p9<0.05);雄性小鼠在第四次和第九次训练的运动速度存在显著低于对照(p4<0.01, p9<0.05)。此外,雄性敲除鼠的基线血糖水平显著低于雄性对照 (p=0.036);禁食16 h,其血糖水平显著高于对照(p<0.05),且禁食前后的血糖改变量显著低于对照(p<0.01)。结论 PAPD4基因缺陷可能影响小鼠的运动功能,并影响雄性小鼠的血糖水平。

关键词: PAPD4, 运动能力, 多聚腺苷酸化, 血糖

Abstract: Objective Learning the influence of PAPD4 gene deletion on mouse behaviors and physiologies. Methods Mice were divided into four groups by sex and genotype: male control, male knockout, female control and female knockout. Open field test, Morris water maze, blood glucose test, blood pressure test, T maze, sucrose preference test and forced swimming test. Results In open field test, the locomotion velocity (p<0.05) and the total distance (p<0.001) of female KO mouse were significantly slower than female controls. In Morris water maze test, the locomotion velocity of KO mouse was lower than normal controls. Specifically, the velocity of female KO mouse was significantly slower than female controls in the sixth and ninth trial (p6<0.05, p9<0.05), and the velocity of male KO mouse was also significantly slower than male controls in the fourth and ninth trial (p4<0.01, p9<0.05). Otherwise, the blood glucose of male KO mouse was significantly lower than male normal controls at ad libitum state(p<0.05); but after 16 hours food deprivation, the blood glucose of male KO mouse was significantly higher than male controls(p<0.05), and analyzing the blood glucose change of before-after feeding, we found the change of male KO mouse was significantly lower than male controls(p<0.01). Conclusion Deleting of PAPD4 gene affects locomotion ability of mouse, and this gene may participate in regulation of blood glucose level in male mouse.

Key words: PAPD4, movement ability, polyadenylation, blood glucose,

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