WNT信号抑制因子DKK3基因对结直肠癌细胞系HCT116迁移与侵袭的影响

基础医学与临床 ›› 2019, Vol. 39 ›› Issue (10): 1437-1443.

WNT信号抑制因子DKK3基因对结直肠癌细胞系HCT116迁移与侵袭的影响

刘桂元,张永慧,庞毅

重庆三峡医药高等专科学校

收稿日期:2019-01-28 修回日期:2019-07-03 出版日期:2019-10-05 发布日期:2019-09-25
通讯作者: 庞毅 E-mail:pbinglan@163.com
基金资助:
重庆市教委科学研究项目;重庆市教委科学研究项目;重庆三峡医药高等专科学校自然科学研究项目

Effect of DKK3 on migration and invasion of colorectal cancer HCT116 cell line

Received:2019-01-28 Revised:2019-07-03 Online:2019-10-05 Published:2019-09-25

摘要/Abstract

摘要： 目的：分析DKK3在结直肠癌组织中的表达及对患者预后的影响，同时探索DKK3对结直肠癌细胞增殖、迁移以及侵袭能力的影响，并对机制进行一定的探索。方法：在线数据库分析DKK3在结直肠癌组织中的表达及其对患者预后的影响。用RNA干扰技术，下调DKK3；用MTT检测细胞增殖，同时运用细胞小室迁移实验检测细胞迁移及侵袭能力，并通过RT-qPCR技术以及Western blot技术检测迁移相关蛋白表达。结果：于正常组织相比，DKK3在结直肠癌组织中的表达显著上调并会导致患者整体预后情况变差(P<0.05)，干涉DKK3后细胞增殖速率明显减缓(P<0.05)，并且细胞迁移以及侵袭能力减弱(P<0.01)。MMP2, MMP7以及MMP9与DKK3的表达呈正相关关系(P<0.001)。在敲低DKK3后，MMP2, MMP7, MMP9以及p-ERK的表达均显著下调(P<0.01)。结论：DKK3在结直肠癌中表达上调，且与患者的预后成负相关，可能通过MAPK/ERK信号通路促进细胞增殖以及迁移能力。

关键词: 关键词：DKK3，结直肠癌，增殖，迁移及侵袭

Abstract: Objective To analyze the expression of DKK3 in colorectal cancer tissues and its effect on the prognosis of patients. To explore the effect of DKK3 on proliferation, migration and invasion ability of colorectal cancer cells, and indicate the mechanism. Methods The expression of DKK3 in colorectal cancer tissues and its effect on the prognosis of patients were analyzed by online database. The expression of DKK3 was down-regulated using shRNA. Cell proliferation was detected by MTT assay. In addition, transwell assay was performed to determine cell migration and invasion. Meanwhile, the expression levels of MMP2, MMP7, MMP9 were detected by RT-qPCR assays and western blot assays. Results The expression of DKK3 in colorectal cancer tissue was higher than that in normal colorectal tissue, and negatively correlated with patient prognosis (P<0.05). Cell proliferation was reduced in DKK3 knocked down group (P<0.05). Compared with negative group, the ability of migration and invasion was weaker (P<0.01). The expression of MMP2, MMP7 and MMP9 were positively correlated with the expression of DKK3 (P<0.001). In addition, the expression levels of MMP2, MMP7, MMP9 and p-ERK were decreased significantly in DKK3 down-regulation group (P<0.01). Conclusions DKK3 is up-regulated in colorectal cancer and negatively correlated with patient prognosis, and it may promote the proliferation and migration of cells through MAPK/ERK signaling pathway.

Key words: Key words：DKK3、colorectal cancer、proliferation、migration and invasion

刘桂元张永慧庞毅. WNT信号抑制因子DKK3基因对结直肠癌细胞系HCT116迁移与侵袭的影响[J]. 基础医学与临床, 2019, 39(10): 1437-1443.