关键词: 脱细胞软骨基质, 软骨细胞, 人胰岛素样生长因子1

Abstract: Objective To research the feasibility of acelluar cartilaginous matrix as a natural material and the effect of human insulin-like growth factor 1 on the proliferation and adhesiveness of chondrocytes on the acelluar cartilaginous matrix. Methods The chondrocytes were extracted from rabbit’s ears cartilage.The third passage chondrocytes were identified with toluidine blue staining and collagen II immunofluorescence staining.The cartilage of rabbit’s knee joint was taken for decellularization.And the acelluar cartilaginous matrix was stained with hematoxylin-eosin staining and Masson staining.It was observed with scanning electron microscopy and detect the porosity.Then using CCK-8 (Cell Counting Kit) to detect the acelluar cartilaginous matrix extracts’ toxicity of 25%, 50%, 100% concentration. Inoculating chondrocytes into the acelluar cartilaginous matrix. In the experimental group, DMEM medium containing 57.14 μg/L human insulin-like growth factor 1 was added.After 7 days,observing chondrocytes with scanning electron microscopy. Results 1) The acelluar cartilaginous matrix was loose and porous.The pore size was (3.48±1.39) μm and the porosity was (90.13±2.52)% under the electron microscope. 2) The experimental group showed that the chondrocytes were growing quickly and they were entered the material and connected with each other.They occupied most of the cavity.The chondrocytes in control group was growing slower than the experimental group.And a large amount of cells clustered on the surface of the material,only a small amount of cells entered the cavity. Conclusions The acelluar cartilaginous matrix is a reliable natural material for repairing after articular cartilage injury. Moreover, human insulin-like growth factor 1 can increase the proliferation and adhesiveness of chondrocytes on the acelluar cartilaginous matrix.

Key words: Acelluar cartilaginous matrix, Chondrocytes, Human insulin-like growth factor 1