过表达骨形成发生蛋白4降低人胶质母细胞瘤系U251的侵袭

基础医学与临床 ›› 2019, Vol. 39 ›› Issue (1): 76-80.

过表达骨形成发生蛋白4降低人胶质母细胞瘤系U251的侵袭

王璨,喻军华

鄂州市中心医院

收稿日期:2018-07-18 修回日期:2018-11-05 出版日期:2019-01-05 发布日期:2018-12-28
通讯作者: 喻军华 E-mail:hbezyjh@126.com

Over-expression of bone morphogenetic protein 4 regulates invasion of human glioblastoma cell line U251

wang can¹,

Received:2018-07-18 Revised:2018-11-05 Online:2019-01-05 Published:2018-12-28

摘要/Abstract

摘要： 目的：探讨骨形成发生蛋白4(BMP4)对人胶质母细胞瘤细胞（U251）侵袭的影响。方法：将U251细胞，通过转染高表达BMP4的质粒及敲低BMP4的siRNA, 分为myc组、myc-BMP4组、siCtrl组、siBMP4组；用划痕实验、荧光定量PCR与免疫印迹技术，检测高/低表达BMP4后，细胞侵袭E-cadherin及Snail1mRNA和蛋白表达。结果：高表达BMP4降低细胞侵袭（P＜0.01），低表达BMP4增加细胞侵袭。高表达BMP4后，Snail1信号通路靶基因E-cadherin表达量明显升高（P＜0.01）；低表达BMP4后，E-cadherin表达量明显降低（P＜0.01）；高表达BMP4 时，Snail1表达量明显降低（P＜0.01）。结论：BMP4 可以通过负性调节Snail1信号通路调节细胞侵袭。

关键词: 侵袭, BMP4, Snail1

Abstract: Objective To investigate the effect of BMP4 on glioblastoma cell invasion and its mechanism. Methods In human malignant glioma cells U251, myc, myc-BMP4, siCtrl, siBMP4 was transfected into cells, and changes on the invasive ability of cells with high and low BMP4 expression were detected by Scratch wound healing assay, In mechanism study, RT-PCR and western blot assay were used to detect the expression of E-cadherin and Snail1 in four groups. Results Wound healing results showed that high BMP4 expression in U251 cells decreased cell invasion（P＜0.01）, and low BMP4 expression increased cell invasion; RT-EPCR and Western blot assay results showed that high BMP4 expression significantly increase E-cadherin expression（P＜0.01）, low BMP4 expression significantly decrease E-cadherin expression（P＜0.01）, when BMP4 expression was high, Snail1 protein expression was decreased（P＜0.01）. Conclusion: BMP4 can regulate GBM cell invasion through negative regulation of Snail1 signaling pathway.

Key words: invasion, BMP4, snail1

王璨喻军华. 过表达骨形成发生蛋白4降低人胶质母细胞瘤系U251的侵袭[J]. 基础医学与临床, 2019, 39(1): 76-80.

wang can. Over-expression of bone morphogenetic protein 4 regulates invasion of human glioblastoma cell line U251[J]. Basic & Clinical Medicine, 2019, 39(1): 76-80.

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