高氧降低不同日龄新生大鼠肠道干细胞Bmi1和Lgr5的表达

基础医学与临床 ›› 2019, Vol. 39 ›› Issue (1): 22-26.

高氧降低不同日龄新生大鼠肠道干细胞Bmi1和Lgr5的表达

娄文静,刘冬妍

中国医科大学附属盛京医院

收稿日期:2018-05-31 修回日期:2018-07-25 出版日期:2019-01-05 发布日期:2018-12-28
通讯作者: 刘冬妍 E-mail:liudy19701010@sina.com
基金资助:
新生大鼠高氧动物模型肠黏膜损伤机制研究;研究暴发性肝衰竭并发自发性腹膜炎肠道树突状细胞变化机制;盛京自由研究者

Hyperoxia reduces the expression of Bmi1 and Lgr5 in intestinal stem cells of newborn rats

¹,Liu Dongyan

Received:2018-05-31 Revised:2018-07-25 Online:2019-01-05 Published:2018-12-28
Contact: Liu Dongyan E-mail:liudy19701010@sina.com

摘要/Abstract

摘要： 目的探讨高氧治疗对不同日龄新生大鼠肠道干细胞Bmi1和Lgr5表达的影响。方法将新生大鼠随机分为正常空气对照组和高氧组（85% O2），在不同日龄（3天、7天、10天、14天）将其处死，收集肠道标本，采用免疫组织化学方法、蛋白免疫印迹方法（Western Blot）及实时荧光定量PCR（RT-PCR）方法检测肠道干细胞Bmi1和Lgr5表达的变化。结果与相对应的不同日龄正常对照组相比,高氧组Bmi1和Lgr5的蛋白及mRNA表达水平均显著下降( P＜0.05)。结论不同日龄新生大鼠在高氧环境中肠道干细胞Bmi1和Lgr5表达水平下降,由此推测高氧影响新生大鼠肠道干细胞的生长发育，这可能是高氧损伤肠道的根源。

关键词: 关键词：高氧, 肠道干细胞, Bmi1, Lgr5

Abstract: Objective To investigate the effect of hyperoxia treatment on the expression of Bmi1 and Lgr5 in intestinal stem cells of neonates with different ages. Methods Neonatal rats were randomly divided into normal air control group and hyperoxia group (85% O2) and sacrificed at different ages (3 days, 7 days, 10 days, 14 days).Intestinal specimens were collected, the expression of Bmi1 and Lgr5 of its intestinal stem cells was detected by immunohistochemistry, Western blott, and real-time PCR. Results The protein and mRNA expression levels of Bmi1 and Lgr5 in the hyperoxia group were significantly lower than those in the normal control group at different ages (P<0.05).Conclusions The expression of Bmi1 and Lgr5 in the intestinal stem cells of newborn rats at different ages was decreased in hyperoxia environment. Therefore, hyperoxia is considered to affect the growth and development of intestinal stem cells in neonatal rats, which may be the reason of hyperoxia-induced intestinal damage.

Key words: Key words：hyperoxia, intestinal stem cells, Bmi1, Lgr5

中图分类号:

娄文静刘冬妍. 高氧降低不同日龄新生大鼠肠道干细胞Bmi1和Lgr5的表达[J]. 基础医学与临床, 2019, 39(1): 22-26.

Liu Dongyan. Hyperoxia reduces the expression of Bmi1 and Lgr5 in intestinal stem cells of newborn rats[J]. Basic & Clinical Medicine, 2019, 39(1): 22-26.

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