肥胖抑制素减轻高糖致大鼠INS1细胞的凋亡

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (1): 7-12.

肥胖抑制素减轻高糖致大鼠INS1细胞的凋亡

徐敏¹,王威¹,姜方旭²,乔虹³,张玲¹

1. 哈尔滨医科大学附属二院
2. Islet Cell Development Program, Harry Perkins Institute of Medical Research, Nedlands
3. 哈尔滨医科大学附属第二医院内分泌科

收稿日期:2016-06-16 修回日期:2017-02-06 出版日期:2018-01-05 发布日期:2017-12-26
通讯作者: 王威 E-mail:wwei19742007@hotmail.com
基金资助:
国家自然科学基金面上项目;黑龙江省自然科学基金项目;黑龙江省教育厅科学技术研究项目;哈医大二院博士基金

Obestatin attenuates apoptosis induced by high glucose in INS1 cells

Received:2016-06-16 Revised:2017-02-06 Online:2018-01-05 Published:2017-12-26

摘要/Abstract

摘要： 目的探讨肥胖抑制素（OB）减轻高糖致大鼠胰岛细胞系INS1细胞的凋亡作用。方法 INS1细胞在不同糖浓度环境下培养24 h，用MTT法检测INS1细胞存活率和增殖。Hoechst 33258细胞核染色法检测细胞核形态学。酶标法检测caspase-3活性及OB的保护作用是否涉及了PI3K；real-time PCR法检测FOXO1、SREBP1C、Bax和PDX-1mRNA的表达。结果在高糖条件下，OB促进INS1细胞的增殖，在100 nmol/L浓度时能最大程度促进INS1细胞增殖（与对照组、高糖组相比P<0.01）。OB能减轻高糖诱导的凋亡(P<0.01)。在高糖组FOXO1、SREBP1c和Bax基因表达较对照组增多，PDX-1、表达减少；反之在OB干预的高糖组FOXO1、SREBP1c和Bax表达减少，PDX-1表达增多。结论OB能够减轻高糖致大鼠INS1细胞的损伤作用。

关键词: 肥胖抑制素, 糖毒性, β细胞, 保护作用

Abstract: Objective To investigate the effect of obestatin on the apoptosis of rat pancreatic islet cell line INS1 induced by high glucose.Methods INS1 cells were cultured in different concentration of glucose.The survival rate and proliferation of INS1cells detected by MTT method;Hoechst 33258 nuclear staining was used to detect nuclear morphology. Caspase-3 method was used to study the relationship between the protective effect of obestatin and the PI3K pathway; Finally，using real time PCR detection of FOXO1and SREBP1c, Bax, PDX-1expression, to further clarify the protective effect of obestatin on cells. Results Under high glucose condition,obestatin promoted the proliferation of INS1 cells at 100nmol/L,and promoted the proliferation of INS1 cells significantly(P<0.01 compared with the control group and high glucose group).Obestatin can reduce high glucose-induced apoptosis(P<0.01).The expressions of FOXO1,SREBP1c,Bax and PDX-1 were decreased,while the expressions of FOXO1,SREBP1c,Bax and PDX-1 were increased in the high glucose group; Conclusions OB can attenuate the injury of INS1 cells induced by high glucose in rats.

Key words: Obestatin, Glucotoxicity, β cells, protection

徐敏王威姜方旭乔虹张玲. 肥胖抑制素减轻高糖致大鼠INS1细胞的凋亡[J]. 基础医学与临床, 2018, 38(1): 7-12.

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