基础医学与临床 ›› 2017, Vol. 37 ›› Issue (8): 1094-1097.

• 研究论文 • 上一篇    下一篇

葛根素减弱ox-LDL诱导HUVECs组织因子及其抑制物的表达

邓华菲1,李坚2,周琴2,谭玉林1,谢明3,韩瑛4,郭皖北1   

  1. 1. 湘南学院
    2. 湘南学院基础医学部
    3. 湘南学院病理学教研室
    4. 湘南学院基础医学院
  • 收稿日期:2016-12-05 修回日期:2017-03-22 出版日期:2017-08-05 发布日期:2017-07-17
  • 通讯作者: 郭皖北 E-mail:2228720587@qq.com
  • 基金资助:
    湖南省教育厅高校创新平台开放基金项目;湘南学院科研基金资助;湖南省重点建设学科资金资助;湘南学院重点建设学科资金资助

Puerarin attenuates the expression of HUVECs tissue factor and its inhibitor induced by ox-LDL

  • Received:2016-12-05 Revised:2017-03-22 Online:2017-08-05 Published:2017-07-17

摘要: 目的 探讨葛根素(puerarin)对氧化型低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVECs)组织因子(TF)和组织因子途径抑制物(TFPI)表达的影响。方法 用不同浓度葛根素和50 mg/L ox-LDL共同孵育HUVECs,实时荧光定量PCR和Western blot法分别检测TF和TFPI mRNA和蛋白表达。 结果 与对照组相比, ox-LDL明显诱导HUVECs TF mRNA和蛋白表达 (P<0.01);而明显抑制TFPI mRNA 和蛋白表达(P<0.01);50、100和200 ?mol/L葛根素呈剂量依赖性降低ox-LDL对TF mRNA和蛋白的诱导作用(P<0.01),减弱ox-LDL对TFPI mRNA和蛋白的抑制作用(P<0.01)。结论 葛根素减轻了ox-LDL对HUVECs TF和TFPI mRNA及蛋白表达的诱导。

关键词: 葛根素, ox-LDL, 组织因子, 组织因子途径抑制物, 人血管内皮细胞

Abstract: Objective To investigate the effects of puerarin on the expression of human umbilical vein endothelial cells (HUVECs) tissue factor (TF) and tissue factor pathway inhibitor (TFPI) induced by oxidized low-density lipoprotein (ox-LDL). Methods After HUVECs were incubated with different concentrations of puerarin and 50 mg/L ox - LDL, the expression of TF and TFPI mRNA and protein were detected by real-time fluorescent quantitative PCR and Western blot respectively. Results Compared with control, treatment with ox-LDL caused the augment of TF mRNA and protein expression (P<0.01), and the decrease of TFPI mRNA and protein expression. However, 50, 100, and 200 ?mol/L puerarin blunted the augment of TF mRNA and protein expression and weakened the inhibition of TFPI mRNA and protein expression induced by ox-LDL(P<0.01). Conclusion Puerarin reduced HUVECs TF and TFPI mRNA and protein induced by ox-LDL.

Key words: puerarin, oxidative low-density lipoprotein, tissue factor, tissue factor pathway inhibitor, vascular endothelial cells