慢性间歇性低氧对大鼠肾脏GLUT-2、IRS-2表达的影响

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (2): 206-210.

慢性间歇性低氧对大鼠肾脏GLUT-2、IRS-2表达的影响

余维,刘霞,骆小华,李兵

重庆医科大学附属第一医院

收稿日期:2016-09-18 修回日期:2016-11-01 出版日期:2017-02-05 发布日期:2017-01-16
通讯作者: 李兵 E-mail:dclibing@sina.com
基金资助:
国家临床重点专科建设项目经费基金资助;重庆市卫生局重点科研项目

Effects of chronic intermittent hypoxia on the expressions of glucose transporter 2 and insulin receptor substrate 2 in rat kidney

Received:2016-09-18 Revised:2016-11-01 Online:2017-02-05 Published:2017-01-16

摘要/Abstract

摘要： 目的探讨慢性间歇性低氧及复氧对大鼠肾脏葡萄糖转运蛋白2（GLUT-2）、胰岛素受体底物2（IRS-2）表达的影响。方法将大鼠分为对照组（control），慢性间歇性低氧组（CIH），慢性间歇性低氧后复氧组（RH）,建立慢性间歇性低氧的动物模型，抽取动脉血立即行血气分析，过氧化物酶法检测血清中血糖及放射免疫法检测大鼠血清胰岛素。取出大鼠肾脏组织，qPCR法检测大鼠肾脏GLUT-2、IRS-2的mRNA表达，Western blot 法检测GLUT-2 、IRS-2蛋白表达；免疫组化法检测大鼠肾脏GLUT2 、IRS-2蛋白表达。结果 control组大鼠血氧饱和度≥95%，CIH组的血氧饱和度≤85%,RH组血氧饱和度≥86%；CIH组大鼠空腹血糖、血清胰岛素及胰岛素抵抗指数较control组、RH组明显升高（P<0.05）；RH组高于control组（P<0.05）；GLUT-2、IRS-2的mRNA及蛋白表达较control组和RH组升高（P<0.05）；RH组高于control组（P<0.05）。结论慢性间歇性低氧升高大鼠空腹血糖，上调大鼠肾脏GLUT-2、IRS2的表达，增强胰岛素抵抗，降低胰岛素敏感性。

关键词: 慢性间歇性低氧, 胰岛素抵抗, 糖代谢, 葡萄糖转运蛋白2, 胰岛素受体底物2

Abstract: Objective To investigate the effects of chronic intermittent hypoxia and on GLUT-2, IRS-2 expression in the rat kidney. Methods Rats were randomly divided into control group (control), chronic intermittent hypoxia group (CIH), chronic intermittent hypoxia reoxygenation group (RH), the establishment of chronic intermittent hypoxia animal models,arterial blood gas analysis were extracted immediately.Serum glucose was measured by peroxidase and serum insulin was detected by radioimmunoassay. Remove the kidney tissue of rats ,protein expression of GLUT-2, IRS-2 were detected with Western Blot and immunohistochemical,mRNA expression of GLUT-2、IRS-2 were observed by qPCR . Results Oximetry in the control group was ≥95%, oxygen saturation in the CIH group was ≤85%, oxygen saturation in the RH group was ≥86%; fasting blood glucose , serum insulin and insulin resistance index in the CIH group were significantly higher than the control group and RH group (P <0.05); RH group was higher than control group (P <0.05). protein and mRNA expression of GLUT-2、 IRS-2 in the CIH group were higher than the control group and RH group (P <0.05); RH group was higher than control group (P <0.05). Conclusion Chronic intermittent hypoxia can increase blood glucose，upregulates the expression of GLUT-2, IRS2 in the rat kidney and enhance insulin resistance and decreased insulin sensitivity.

Key words: chronic intermittent hypoxia, insulin resistance, glucose metabolism, glucose transporter 2, insulin receptor substrate 2

中图分类号:

R335+.9

余维刘霞骆小华李兵. 慢性间歇性低氧对大鼠肾脏GLUT-2、IRS-2表达的影响[J]. 基础医学与临床, 2017, 37(2): 206-210.

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