基础医学与临床 ›› 2016, Vol. 36 ›› Issue (9): 1237-1241.

• 研究论文 • 上一篇    下一篇

雌激素对去势大鼠髁突软骨雌激素受体基因表达的影响

关敏,张跃蓉,范迪,刘忠林   

  1. 遵义医学院
  • 收稿日期:2015-07-06 修回日期:2016-01-04 出版日期:2016-09-05 发布日期:2016-08-30
  • 通讯作者: 张跃蓉 E-mail:495454380@qq.com
  • 基金资助:
    贵州省科学技术基金项目

Effects of estrogen on estrogen receptors gene expression of condylar cartilage of ovariectomized rats

  • Received:2015-07-06 Revised:2016-01-04 Online:2016-09-05 Published:2016-08-30

摘要: 目的 探讨不同浓度的雌二醇(E2)对去势大鼠髁突软骨雌激素受体(ER)基因ERα和ERβ表达的影响。方法 将SD大鼠分为对照组 (control)、假手术组(sham)、去势组(OVX)、及时和延迟替代治疗组(OVX/17β-E2),每天静脉注射不同浓度雌二醇(5×10、5×102和5×103μg/kg ),阴道上皮脱落细胞涂片监测雌激素水平,用Westernblot和Real time-PCR法检测大鼠颞下颌关节髁突软骨ERα和ERβ的蛋白及mRNA表达。结果 去势大鼠阴道上皮细胞MI指数180/12/8,表明雌激素水平低下(P<0.05)。及时补给生理浓度雌二醇(5×102μg /kg)能维持髁突软骨ER于正常范围,延迟补给生理浓度雌二醇不能恢复或改善ER表达;及时和延迟补给高浓度(5×103μg /kg)及低浓度(50μg/kg)E2均抑制ER蛋白和 mRNA合成(P<0.05),且高浓度效应显著。结论 及时补充合理浓度的雌激素对去势大鼠颞下颌关节的正常生理功能具有重要意义。

关键词: 髁突软骨, 雌激素, 雌激素受体

Abstract: Objective This study investigated the role of estrogen receptors in the metabolism of temporomandibular cartilage by applying different concentration of 17β-estradiol (17β-E2) to ovariectomized (OVX) rats.Methods 1.The female SD rats were randomly assigned to the control group,sham group,OVX group and hormone replacement therapy group . Different concentration of 17β-E2 were injected timely and delayed. 2. The change of estrogen level was verified by testing the shape and mature index of vaginal epithelium exfoliated cells, then the ERα and ERβ in rat condylar cartilage were tested by using Western-blot and Real-time PCR analysis subsequently. Results 1)The maure index of vaginal epithelium exfoliated cells of OVX rats was 180/12/8, indicating low levels of estrogen in vivo(P<0.05). 2) ER mRNA could maintain at the normal level when 5×102μg/kg of 17β-E2 were supplemented immediately. Yet this was not the case when 17β-E2 were given delayed. 3. Both 50μg/kg and 5×103μg/kg of 17β-E2 administration can suppress the expression of ER, either in protein or mRNA level, and this inhibitory effect is more obvious in 5×103μg/kg group(P<0.05).Conclusion The biological effects of OVX rats of ER depend on normal physiological concentration and accurate period of estrogen secretion.

Key words: Condylar cartilage, Estrogen, Estrogen Receptor

中图分类号: