富硒灵芝多糖改善高脂饮食诱导的非酒精性脂肪肝大鼠症状

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (9): 1193-1198.

富硒灵芝多糖改善高脂饮食诱导的非酒精性脂肪肝大鼠症状

贾亚敏¹,武俊紫²,胡跃高³,李树德⁴,沈平瑞⁵

1. 太原理工大学
2. 东华大学
3. 中国农业大学
4. 昆明医科大学基础医学院
5. 沈阳康硒生物工程研究所

收稿日期:2015-09-21 修回日期:2015-12-07 出版日期:2016-09-05 发布日期:2016-08-30
通讯作者: 李树德 E-mail:wu70521@126.com
基金资助:
从构筑转录因子HMGA2在胃癌中的调控网络探讨上皮间质转化和肿瘤细胞干性获取之间的关系

Se Ganoderma lucidum polysaccharides ameliorate the symptome of high fat diet induced non-alcoholic fatty liver in disease rats

Received:2015-09-21 Revised:2015-12-07 Online:2016-09-05 Published:2016-08-30

摘要/Abstract

摘要： 目的探讨富硒灵芝多糖对非酒精性脂肪肝病大鼠SREBP-1c及ACCα表达的影响。方法 120只SD大鼠随机分为正常组、高脂饮食组、多烯磷脂酰胆碱组、富硒灵芝多糖低、中和高剂量干预组等6组，每组20只，分别于治疗4和8周后处死大鼠各半，测血清中谷草转氨酶（AST）、谷丙转氨酶（ALT）、总胆固醇（CHOL）和三酰甘油（TG）；用Western blot和RT-PCR检测肝脏组织中SREBP-1c及ACCα蛋白和mRNA表达；其余肝脏行病理学检测。结果富硒灵芝多糖可显著减少肝脏组织中的脂肪颗粒，并降低血清中AST、ALT、CHOL和TG含量，中、高剂量的灵芝多糖处理可显著降低肝脏中SREBP-1c、ACCα mRNA和蛋白的表达（p<0.05）。结论富硒灵芝多糖可缓解高脂饮食诱导的非酒精性脂肪肝大鼠症状。

关键词: 富硒灵芝多糖, 非酒精性脂肪肝, 固醇调节原件结合蛋白-1c, 乙酰辅酶A羧化酶α

Abstract: Objective To investigate the expression of ACCα and SREBP-1c of selenium-enriched Ganoderma lucidum polysaccharides on fatty liver rats model. Methods 120 SD rats were randomly divided into control, high-fat diet, polyene phosphatidylcholine, Se G. lucidum polysaccharides low, medium and high group, after 4 and 8 weeks of treatment the rats were sacrificed, measuring the AST, ALT, CHOL and TG in blood serum, detect SREBP-1c, ACCα mRNA and protein expression by Western Blot and PCR respectively. Results With the increase of treatment dose and treatment time of Se G. lucidum polysaccharides, fatty infiltration of the liver was significantly reduced, fat particles significantly reduced; liver function and blood lipids significantly improved; SREBP-1c, ACCα mRNA and protein expression was significantly decreased. Conclusion Se G. lucidum polysaccharides can relieve the symptoms of non- alcoholic fatty liver disease in rats.

Key words: Se Ganoderma lucidum polysaccharides, nonalcoholic fatty liver, sterol regulatory binding proteins -1c, acetyl coenzyme A carboxylase α

中图分类号:

R575.5

贾亚敏武俊紫胡跃高李树德沈平瑞. 富硒灵芝多糖改善高脂饮食诱导的非酒精性脂肪肝大鼠症状[J]. 基础医学与临床, 2016, 36(9): 1193-1198.

[1]	邢苗苗张晓燕管又飞. 奥贝胆酸的研究进展[J]. 基础医学与临床, 2019, 39(10): 1494-1498.
[2]	贺茜邹秀兰. IKKε影响代谢性疾病的相关机制研究进展[J]. 基础医学与临床, 2015, 35(12): 1686-1690.
[3]	叶瑶于健. 脂联素及基因多态性与非酒精性脂肪肝的研究进展[J]. 基础医学与临床, 2014, 34(9): 1285-1288.
[4]	李欣金爱花黄媛李成浩金海燕. 替米沙坦抑制大鼠非酒精性脂肪肝纤维化[J]. 基础医学与临床, 2014, 34(10): 1321-1326.