基础医学与临床 ›› 2016, Vol. 36 ›› Issue (4): 433-438.

• 研究论文 •    下一篇

小檗碱减轻大鼠心肌缺血再灌注损伤

于立明1,段维勋1,赵国龙2,张秋芳3,王晓武1,金振晓1,俞世强1   

  1. 1. 第四军医大学西京医院
    2. 宁夏医科大学附属医院
    3. 西安市第四医院
  • 收稿日期:2015-07-10 修回日期:2015-11-09 出版日期:2016-04-05 发布日期:2016-03-29
  • 通讯作者: 俞世强 E-mail:shiqiangyu210@126.com
  • 基金资助:
    国家十二五科技支撑计划课题;国家自然科学基金;陕西省社会发展攻关计划;陕西省国际科技合作与交流计划项目;西京医院学科助推计划

Berberine attenuates myocardial ischemia reperfusion injury in rat

  • Received:2015-07-10 Revised:2015-11-09 Online:2016-04-05 Published:2016-03-29
  • Supported by:
    ;National Natural Science Foundation of China

摘要: 目的 探讨小檗碱(BBR)减轻大鼠心肌缺血再灌注(MI/R)损伤的作用及其与JAK2/STAT3信号通路的关系。方法 雄性SD大鼠(140只)随机分为7组 (n = 36):假手术组(Sham)、Sham+BBR组、Sham+AG490(AG,JAK2/STAT3通路抑制剂)组、MI/R+溶剂组(MI/R+V)、MI/R+BBR组、MI/R+BBR+AG组、MI/R +AG组。常规结扎大鼠冠状动脉左前降支行心肌缺血再灌注手术,缺血30 min后再灌注4 h,用Western blot法检测心肌JAK2、STAT3磷酸化水平及心肌凋亡相关蛋白表达,再灌注6 h后检测心肌细胞凋亡率、梗死面积及氧化应激相关指标,再灌注72 h后检测心功能。结果 BBR可显著改善心肌缺血再灌注术后心功能并减轻心肌凋亡及梗死,这种保护作用可被AG阻断(均P < 0.05)。BBR治疗还可显著提高心肌JAK2及STAT3磷酸化水平,抑制凋亡相关信号分子表达,这种作用也被AG阻断(P < 0.05)。结论 BBR可显著减轻大鼠MI/R损伤后心肌梗死及凋亡水平,改善心功能,JAK2/STAT3可能介导此作用。

关键词: 小檗碱,JAK2/STAT3信号通路,心肌缺血再灌注损伤,凋亡,心肌保护

Abstract: Objective To determine the protective effect of berberine (BBR) on myocardial ischemia/reperfusion (MI/R) injury in rats and the role of JAK2/STAT3 signaling pathway in this process. Methods Male Sprague-Dawley rats were randomly divided into 7 groups (n=36): Sham, Sham+BBR, Sham+AG (AG490, the inhibiter of JAK2/STAT3 signaling pathway), MI/R+V (vehicle), MI/R+BBR, Sham+BBR+AG and MI/R+AG, and then exposed to MI/R insult. After 4 h of reperfusion, myocardial p-JAK2/JAK2 and p-STAT3/STAT3 ratios, and the apoptotic related protein expressions were assessed. After 6 h of reperfusion, myocardial apoptotic index and infarct size were evaluated. The cardiac function were measured after72 h of reperfusion. Results BBR treatment significantly improved cardiac functional and reduced cardiomyocytes apoptosis. In addition, BBR treatment also activated JAK2/STAT3 signaling and suppressed apoptotic signaling. However, these protective effects were all inhibited by AG treatment. Conclusion The present experiment showed that BBR attenuated MI/R injury by reducing apoptosis possibly via activating JAK2/STAT3 signaling pathway.

Key words: Berberine, JAK2/STAT3 signaling pathway, myocardial ischemia/reperfusion injury, apoptosis, cardioprotection

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