基础医学与临床 ›› 2015, Vol. 35 ›› Issue (6): 797-801.

• 研究论文 • 上一篇    下一篇

促红细胞生成素抑制TGF-β诱导的大鼠心肌成纤维细胞增殖

王丽萍,王丽君,王小君   

  1. 河北联合大学基础医学院生理学系
  • 收稿日期:2014-10-20 修回日期:2014-12-27 出版日期:2015-06-05 发布日期:2015-05-27
  • 通讯作者: 王丽萍 E-mail:mywlpzjm@163.com

Erythropoietin inhibits rat cardiac fibroblasts proliferation induced by TGF-β

  • Received:2014-10-20 Revised:2014-12-27 Online:2015-06-05 Published:2015-05-27
  • Contact: Li-Ping WANG E-mail:mywlpzjm@163.com

摘要: 目的 探讨促红细胞生成素在大鼠心肌成纤维细胞(CFs)增殖中的作用。方法 培养大鼠CFs。实验分为对照组( Control )、TGF-β刺激组(TGF-β终浓度为5μg/L)和重组人促红细胞生成素(rhEPO,5000U/L)干预组: 1 h后加入TGF-β。24 h后计数细胞并采用MTT法观察细胞增殖;免疫细胞化学及Western blot法检测α-SMA表达,观察细胞转化;羟脯氨酸定量检测细胞胶原含量;Western blot法检测细胞Ⅰ、Ⅲ型胶原蛋白及MMP-2、MMP-9表达。结果 与对照组比较,TGF-β使细胞明显增殖(P<0. 05),Ⅰ、Ⅲ型胶原蛋白合成(P<0. 05)、α-SMA表达(P<0. 05)、细胞MMP-2、MMP-9表达增多( P<0. 05)。使用重组人促红细胞生成素(rhEPO)干预后,CFs增殖、α-SMA表达及Ⅰ、Ⅲ型胶原蛋白合成较TGF-β组均显著降低 (P<0. 05),MMP-2、MMP-9表达进一步增多(P<0. 05)。结论 生理剂量EPO可抑制TGF-β诱导的大鼠CFs增殖、转化及胶原的合成,促进胶原降解。

关键词: 关键词 促红细胞生成素, 心肌纤维化, 心肌成纤维细胞

Abstract: Objective To explore the effect of erythropoietin on the proliferation of rat cardiac fibroblasts (CFs) induced by TGF-β. Methods Cultivated rats CFs were divided into three groups: Control group, TGF-βgroup: the final concentration of TGF-βwas 5 μg/L, recombinant human erythropoietin (rhEPO) treatment group: added TGF-βafter incubation with 5000U/L rhEPO for one hour. After 24 hours, CFs proliferation was determined by MTT assay and cell counting; Immunocytochemistry and western blot were conducted to observeα-SMA expression; The hydroxyproline concentration of CFs was assessed by hydroxyproline kit; CollagenⅠ,Ⅲ and MMP-2,MMP-9 synthesis of CFs were deteched by Western blot. Results Compared with control group, TGF-βpromoted the CFs proliferation and transformation (P<0.05); and TGF-βinduced increase synthesis of collagenⅠand collagen Ⅲ as well as MMP-2 and MMP-9 in CFs (P<0.05); After EPO intervention, CFs proliferation and transformation were decreased (P<0.05), collagenⅠand collagen Ⅲ synthesis were reduced (P<0.05), but the secretion of MMP-2 and MMP-9 were increased (P<0.05). Conclusion Physiological dose EPO inhibits rat CFs proliferation, transformation and collagen production, in addition, EPO promoted collagen degradation.

Key words: Key words: Erythropoietin, myocardial fibrosis, cardiac fibroblasts

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