基础医学与临床 ›› 2015, Vol. 35 ›› Issue (5): 661-664.

• 研究论文 • 上一篇    下一篇

胃癌细胞中长链非编码RNA SNHG5 与miR-26b间的相互作用

李延爽1,赵连梅2,孙佳增1,刘彦信3,郑德先3,史娟3   

  1. 1. 中国医学科学院 北京协和医学院基础学院
    2. 河北医科大学第四医院
    3. 中国医学科学院基础医学研究所
  • 收稿日期:2014-12-24 修回日期:2015-03-12 出版日期:2015-05-05 发布日期:2015-04-28
  • 通讯作者: 史娟 E-mail:shijuantt@163.com
  • 基金资助:
    国家自然基金

Interaction of miR-26b and lncRNA SNHG5 in gastric cancer

  • Received:2014-12-24 Revised:2015-03-12 Online:2015-05-05 Published:2015-04-28

摘要: 目的 探讨胃癌细胞中长链非编码RNA SNHG5与miR-26b 的相互作用及其机制。方法 实时定量 PCR 检测SNHG5在GES-1 胃黏膜正常上皮细胞与胃癌细胞中的表达差异,通过瞬时转染SNHG5 过表达质粒,Taq Man 定量PCR检测MGC-803 细胞中miR-26b 及pri-miR-26b的表达变化,同时瞬时转染miR-26b mimics检测其对SNHG5表达的影响。结果 SNHG5 在胃癌细胞中的表达明显高于胃粘膜正常上皮细胞GES-1(P<0.05),SNHG5 能够抑制miR-26b 与pri-miR-26b的表达(P<0.01),但miR-26b 不能对SNHG5的表达产生影响。结论 SNHG5可抑制miR-26b 的表达,具体作用机制还需要进一步分析。

关键词: 胃癌, SNHG5, miR-26b

Abstract: Objective To explore the interaction and mechanism between miR-26b and lncRNA SNHG5. Methods Quantitative real-time PCR analyzed the differential expression of SNHG5 in normal gastric epithelial cell GES-1 compared with it in gastric cancer cell. Transient transfected MGC-803 cells with SNHG5 overexpressed plasmid and detect the expression level of miR-26b and pri-miR-26b by Taq Man PCR analysis. Meanwhile, transient transfected MGC-803 cells with miR-26b mimics to detect the expression change of SNHG5. Results Expression level of SNHG5 was significantly higher in gastric cancer cells than in normal gastric epithelial cell GES-1 (P<0.05). SNHG5 inhibited the expression of miR-26b and pri-miR-26b(P<0.01), but miR-26b could not influence the expression of SNHG5. Conclusion SNHG5 inhibited the expression of miR-26b, but the mechanism need to be further clarified.

Key words: Gastric cancer, SNHG5, miR-26b

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