关键词: 关键词：小鼠CSE基因, 启动子, 基因序列分析, 转录活性分析

Abstract: Objective The promoter sequences of mouse CSE gene were cloned, and its transcriptional regulatory mechanisms were studied, too. Methods The promoter sequence of CSE gene was cloned from the mouse blood genomic DNA by PCR, and was jointed into the pGL4.12 cloning vector after purification. The sequences of CSE gene promoter were analyzed by a series of bioinformatics software, and the reporter gene activity was tested transient transfection, and promoter activity has been measured and compared to the blank reporter vector in both cell lines. Results There were 25 transcription factor binding sites with more than 92 scores (four GATAs, three SRYs, two USFs, two MZF1s, two v-Mybs, two CdxAs, one TATA, one AML -1 a, one RORalp, one C/EBP, one Nkx-2, one Lyf-1, one N-Myc, one HSF2, and one E2F) by analyzing regulator sequence of CSE gene promoter but CpG island was not detected in CSE regulator region. Conclusion The results provide the experimental and theoretical basis for further study of the transcriptional and expressional regulation mechanism of the CSE gene.

Key words: Key words: mouse CSE gene, promoter, sequence analysis, activity analysis