与泌尿生殖窦间质细胞共培养诱导hUC-MSCs定向分化为前列腺上皮样细胞

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (9): 1175-1178.

与泌尿生殖窦间质细胞共培养诱导hUC-MSCs定向分化为前列腺上皮样细胞

李望,高维强

上海交通大学医学院附属仁济医院

收稿日期:2013-12-19 修回日期:2014-03-28 出版日期:2014-09-05 发布日期:2014-09-02
通讯作者: 高维强 E-mail:wqiangg829@sina.com
基金资助:
国家“973”计划;国家自然科学基金

Directed differentiation of hUC-MSCs into prostate epithelial-like cells when co-cocultutred with urogenital sinus mesenchymal cells

Received:2013-12-19 Revised:2014-03-28 Online:2014-09-05 Published:2014-09-02
Supported by:
National Program on Key Basic Research Project of China;the Chinese National Natural Science Foundation

摘要/Abstract

摘要： 目的研究人的脐带间充质干细胞（hUC-MSCs）与特异性的组织细胞在体外共培养，观察hUC-MSCs是否能够被诱导定向分化。方法原代培养hUC-MSCs和SD大鼠的泌尿生殖窦的间质细胞（rUGSSs）。将细胞消化传代、扩增、鉴定。将两种细胞混合在鼠尾胶中进行三维体外共培养，加雄激素诱导两周后将含有细胞的鼠尾胶做冰冻切片免疫荧光分析前列腺细胞特异性的标记物。结果将hUC-MSCs联合rUGSSs在体外诱导培养两周后，一些细胞表达前列腺上皮的标志物。包括基底细胞的标志物细胞角蛋白5（CK5）和P63蛋白、腔细胞的标志物细胞角蛋白8(CK8)染色阳性。结论 UC-MSCs与rUGSSs在体外共培养后，可以诱导为前列腺上皮样细胞。

关键词: 脐带, 间充质干细胞, 微环境, 分化

Abstract: Objective The aim of this study is to explore whether human umbilical cord-derived mesenchymal stem cells （hUC-MSCs）can be induced to undergo directed differentiation when co-cultured with tissue-specific cells in a three-dimensional gel. Methods Human UC-MSCs and rat urogenital sinus mesenchymal cells（rUGSSs） were isolated and cultured. The two kinds of cells were subcultured, amplified and characterized. hUC-MSCs were co-cultured with rUGSSs in a 3-dimensional collagen gel in vitro. Results Here we report that when hUC-MSCs were co-cultured with rUGSSs in a 3-dimensional collagen gel in vitro, they could differentiate into prostate epithelial-like cells. These prostatic epithelial-like cells could be verified with prostate epithelial cell markers. Conclusion hUC-MSCs could be induced into prostate epithelial-like cells by co-culture with rUGSSs. rUGSSs might provide a stem cell niche that promotes the hUC-MSCs to undergo a directed differentiation into cells of a specific type of tissue cells.

Key words: umbilical cord, mesenchymal stem cells, microenvironment, differentiation

中图分类号:

Q254

李望高维强. 与泌尿生殖窦间质细胞共培养诱导hUC-MSCs定向分化为前列腺上皮样细胞[J]. 基础医学与临床, 2014, 34(9): 1175-1178.

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