基础医学与临床 ›› 2014, Vol. 34 ›› Issue (9): 1171-1174.

• 研究论文 • 上一篇    下一篇

环孢素A改善创伤失血性休克大鼠血管及器官功能

雷艳,董兆君   

  1. 第三军医大学
  • 收稿日期:2013-09-09 修回日期:2013-11-25 出版日期:2014-09-05 发布日期:2014-09-02
  • 通讯作者: 董兆君 E-mail:zhaojundongcq@163.com
  • 基金资助:
    家族性高胆固醇血症相关功能突变的组学筛查及基因敲除小鼠模型的建立

Cyclosporin improves the vascular and organ function after undergo traumatic hemorrhagic shock

  • Received:2013-09-09 Revised:2013-11-25 Online:2014-09-05 Published:2014-09-02

摘要: 目的 观察环孢素A(CsA)对创伤失血性休克大鼠血管收缩/舒张功能、重要器官血流量和功能及线粒体功能的影响,以探讨CsA发挥抗休克作用的途径。 方法 采用大鼠创伤失血性休克模型(维持血压40 mmHg 3 h,伴左侧股骨折断),分为6组:对照组、休克组、乳酸林格氏液(LR)组、CsA 1、5和10 mg/kg组,每组n=8。在休克模型建立后,按照分组分别输注LR或CsA,然后用离体血管张力测定技术观察肠系膜上动脉(SMA)血管收缩和舒张功能,用激光多普勒血流监测仪检测肝/肾血流量,用生化分析法测定肝肾功能,用线粒体呼吸控制率检测法测定肝、肾、肠的线粒体功能。 结果 休克损伤使大鼠SMA的收缩反应性和舒张反应性都明显降低,休克组血管最大收缩反应和最大舒张率分别为正常组的59.3%和71.8%(P<0.01),CsA可明显恢复休克大鼠的血管收缩/舒张功能,其中CsA 5 mg/kg组的最大收缩反应性和最大舒张率分别恢复至正常组的93.4%和91.1%,显著高于LR组(P<0.01~0.05)。同时,CsA 5 mg/kg也明显提高了休克动物肝/肾组织血流量,改善肝肾功能和肝、肾、肠的线粒体功能(P<0.01~0.05)。 结论 CsA可恢复休克后血管功能,提高器官血流量,改善器官和线粒体功能,发挥对创伤休克的治疗作用。

关键词: 环孢素A, 创伤, 失血性休克, 器官功能

Abstract: Objective To observe the effect of cyclosporin A (CsA) on the vasoconstriction/vasodilation of the superior mesenteric artery (SMA), vital organ and mitochondrial function after traumatic hemorrhagic shock in rats. Methods With the traumatic hemorrhagic shock model of rats (blood pressure maintained at 40 mmHg and femur was fractured), rats were divided into six groups: sham-operated, shock, LR, CsA 1, 5, 10 mg/kg (n=8/group). After shock, rats received an infusion of LR or CsA, then the vascular constriction/relaxation of SMA was observe with isolated organ perfusion system, the blood flow in liver and kidney was measured by a laser doppler blood flowmeter, the function of the liver and kidney was measured using biochemical analysis, and the mitochondrial function of the liver, kidney and small intestine was examined by the respiration control rate method. Results After shock, both the vascular constriction reactivity and the vasodilator reactivity were significantly decreased. CsA markedly restored the decreased constriction and relaxation of SMA after shock (P<0.01~0.05), and 5 mg/kg of CsA showed a better effect. CsA (5 mg/kg) also improved tissue blood flow, organ and mitochondrial function in shock rats (P<0.01~0.05). Conclusions CsA has good effects on traumatic hemorrhagic shock through improving vascular function, increasing tissue blood flow, protecting organ and mitochondrial function.

Key words: cyclosporin A, trauma, hemorrhagic shock, organ function

中图分类号: