基础医学与临床 ›› 2014, Vol. 34 ›› Issue (6): 753-756.

• 研究论文 • 上一篇    下一篇

侵袭性乳腺癌GATA5基因启动子甲基化的临床意义

王达,刘秋均,张雪梅,李宏江,李昌龙   

  1. 四川大学华西基础医学与法医学院生物化学与分子生物学教研室
  • 收稿日期:2014-02-18 修回日期:2014-04-13 出版日期:2014-06-05 发布日期:2014-05-26
  • 通讯作者: 李昌龙 E-mail:changlongli@scu.edu.cn
  • 基金资助:
    国家自然科学基金

Promoter hypermethylation of GATA5 and its impact on clinical outcome in invasive breast cancer

  • Received:2014-02-18 Revised:2014-04-13 Online:2014-06-05 Published:2014-05-26
  • Contact: Changlong Li E-mail:changlongli@scu.edu.cn
  • Supported by:
    the Chinese National Natural Science Foundation

摘要: 目的 探讨浸润性乳腺癌(IBC)组织中GATA结合蛋白5(GATA5)基因启动子甲基化与mRNA表达的关系及其意义。方法 用甲基化特异性PCR(MSP)技术检测在62名患者的IBC及配对的癌旁正常组织(MNT)中GATA5基因启动子的甲基化状态。在其中48名患者的浸润性乳腺癌(IBC)及配对的癌旁正常组织(MNT)中,用反转录实时荧光定量PCR (qRT-PCR)检测GATA5 mRNA的表达。结果 GATA5基因在IBC中的甲基化率为48.4% (30/62) (P < 0.001),MNT中为非甲基化。IBC中GATA5 mRNA表达水平显著低于MNT (P < 0.001)。相比于MNT,甲基化的IBC中GATA5基因具有显著较低的mRNA表达(P < 0.001)。同时GATA5基因在甲基化的IBC组中比在非甲基化的IBC组中具有显著较低的表达倍数变化值(P < 0.05)。结论 GATA5基因启动子甲基化很可能引起其转录失活,与IBC的发生、发展密切相关。

关键词: 关键词:浸润性乳腺癌, 甲基化, mRNA表达, GATA5

Abstract: Objective To detect promoter methylation status and mRNA expression level of GATA5 and to explore the relationship between methylation and mRNA expression in invasive breast cancers (IBC). Methods Promoter methylation analysis of GATA5 was performed by methylation-specific PCR (MSP) in IBC and matched normal tissues (MNT) from 62 patients. We examined expression profile of GATA5 using quantitative real-time PCR (qRT-PCR) analysis in 48 of 62 patients. Results Methylation of GATA5 was only present in IBC (48.4% (30 of 62)) (P < 0.001). mRNA expression of GATA5 in IBC showed the lower level than that in MNT (P < 0.001). For GATA5, methylated IBC only showed significantly lower expression values compared to MNT (P < 0.001). Tumors with methylated GATA5 showed significantly less fold change of GATA5 than tumors without methylation (P < 0.05). Conclusions Methylation of GATA5 maybe is one important mechanism of the gene transcriptional inactivation, and is associated with the formation and development of IBC.

Key words: Key words: invasive breast cancers, methylation, mRNA expression, GATA5

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