外源性BMMSCs通过改善内源性BMMSCs成骨分化能力缓解去卵巢大鼠骨质疏松

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (5): 610-614.

外源性BMMSCs通过改善内源性BMMSCs成骨分化能力缓解去卵巢大鼠骨质疏松

帅逸¹,于洋²,邵秉一²,常鹤然³,张立超³,廖立⁴,金岩⁴

1. 中国人民解放军第四军医大学口腔医学院
2. 重庆医科大学附属口腔医院
3. 佳木斯大学附属第二口腔医院
4. 中国人民解放军第四军医大学口腔医院

收稿日期:2013-10-31 修回日期:2014-01-13 出版日期:2014-05-05 发布日期:2014-04-28
通讯作者: 金岩 E-mail:yanjinfmmu@139.com
基金资助:
国家重点基础研究发展计划(973)

Exogenous BMMSCs ameliorate osteoporosis of ovariectomized rat via improving osteogenesis of endogenous BMMSCs

Received:2013-10-31 Revised:2014-01-13 Online:2014-05-05 Published:2014-04-28

摘要/Abstract

摘要： 目的观察系统注射骨髓间充质干细胞（BMMSCs）是否通过改善内源性BMMSCs成骨分化能力缓解去卵巢大鼠骨质疏松。方法 12只雌性SD大鼠随机分为假手术（Sham）组、去卵巢（OVX）组、高剂量（High-dose）组和低剂量（Low-dose）组。建立OVX和Sham模型。术后24h，高、低剂量组分别经尾静脉注射1.5×107和0.375×107 cells/kg细胞。microCT扫描股骨近端。ALP和茜素红染色检测BMMSCs成骨能力，RT-PCR检测成骨相关基因。结果 1）OVX组的骨密度（BMD）和骨小梁数量（Tb.N）低于Sham组（P＜0.05），OVX组内源性BMMSCs的成骨能力弱于Sham组（P＜0.05）。2）高剂量组的BMD和Tb.N高于OVX组（P＜0.05）。高、低剂量组内源性BMMSCs的成骨能力均强于OVX组（P＜0.05），高剂量组强于低剂量组（P＜0.05）。结论系统注射BMMSCs通过改善OVX大鼠内源性BMMSCs的成骨分化能力，缓解OVX大鼠骨质疏松。

关键词: 关键词：系统注射, 骨髓间充质干细胞, 成骨分化, 骨质疏松

Abstract: Objective To witness whether systemic administration of bone marrow mesenchymal stem cells (BMMSCs) can preventing osteoporosis of ovariectomized rat via ameliorating osteogenesis of endogenous BMMSCs. Methods Twelve female Sprague-Dawley rats were randomly divided into group Sham, OVX, High-dose and Low-dose. Established OVX and Sham model. 24 hours after operation, rats in group high-dose and low-dose were systemically injected BMMSCs via tail vein at the dose of 1.5×107 and 0.375×107 cells/kg, representatively. Proximal femurs were analysed by MicroCT. Osteogenesis of BMMSCs was examined by ALP, alizarin red staining and real-time PCR. Results Bone mineral density (BMD) and trabecular bone number (Tb.N) of group OVX were lower than those from group Sham. BMD and Tb.N of group High-dose were higer than group OVX. Compared to group OVX, osteogenesis of endogenous BMMSCs derived from group High-dose and Low-dose were much better, and that from group High-dose turned out to be better than group Low-dose. Conclusion It is effective to relieve symptoms of osteoporosis in ovariectomized rats through treating them with exogenous BMMSCs by the way of rescuing osteogenesis of endogenous BMMSCs.

Key words: Key words: systemic injection, bone marrow mesenchymal stem cells, osteogenesis, osteoporosis

中图分类号:

R322.7+1

帅逸于洋邵秉一常鹤然张立超廖立金岩. 外源性BMMSCs通过改善内源性BMMSCs成骨分化能力缓解去卵巢大鼠骨质疏松[J]. 基础医学与临床, 2014, 34(5): 610-614.

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