N-cadherin对大鼠脊髓损伤后移植神经干细胞存活、增殖与迁移的影响

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (3): 372-375.

N-cadherin对大鼠脊髓损伤后移植神经干细胞存活、增殖与迁移的影响

颜建辉,黄丽娟,杨柳,华力明

湘南学院附属医院

收稿日期:2013-02-18 修回日期:2013-06-26 出版日期:2014-03-05 发布日期:2014-02-27
通讯作者: 颜建辉 E-mail:chenhaojingzhou@163.com
基金资助:
湖南省科技厅自然科学基金

Effect of N-cadherin on survival，proliferation and migration of neural stem cells transplanted into injured spinal cord of rats

Received:2013-02-18 Revised:2013-06-26 Online:2014-03-05 Published:2014-02-27

摘要/Abstract

摘要： 目的探讨神经钙黏蛋白(N-cadherin)对大鼠脊髓损伤(SCI)后移植神经干细胞(NSCs)存活、增殖与迁移的影响。方法 60只SD大鼠随机分为对照组、模型组、移植 1组和移植 2组。横断T12复制SCI模型。移植 1组行原代培养NSCs移植；以lentivirus病毒为载体将N-cadherin转染至原代培养NSCs再植入移植 2组。BBB评分评定大鼠神经功能。术后5、10和15d免疫荧光检测NSCs存活、增殖和迁移。结果模型组BBB评分显著低于对照组(P<0.05)；各移植NSCs组BBB评分均高于模型组(P<0.05)，但以移植2组评分最高(P<0.05)；移植2组各时间点巢蛋白(Nestin)阳性细胞数量均显著多于移植1组(P<0.05)，以15d 的数量最多(P<0.05)；移植2组各时间点Nestin阳性细胞迁移距离显著长于移植1组。结论 N-cadherin可以促进NSCs的存活、增殖与迁移，以修复SCI大鼠的神经功能。

关键词: 神经钙黏蛋白, 脊髓损伤, 神经干细胞

Abstract: Objective To investigate the effect on survival，proliferation and migration of NSCs transplanted into SCI of rats. Methods 60 SD rats were divided into control group, SCI group, transplanting group 1 and transplanting group 2. Using lentivirus virus as a vector, N-cadherin was transfected into primary cultured NSCs to implant in transplanting group 2. Neurological function were evaluated by BBB score. Immumofluorescence was used to detected the survival，proliferation and migration of NSCs at 5, 10 and 15d. Results BBB score of SCI group was lower than control group. BBB score in two transplanted groups increased significantly(P<0.05), with most evident in transplanting group 2(P<0.05). The number of Nestin-positive cells in transplanting group 2 was more than that in transplanting group 1 at the corresponding time point(P<0.05). The number of Nestin-positive cells at 15d was the most among three time points(P<0.05). The immigrating distance in transplanting group 2 was longer(P<0.05)．Conclusion N-cadherin can promote the survival, proliferation and migration of NSCs in SCI rats to repair neurological function.

Key words: N-cadherin, neural stem cells, spinal cord injury

颜建辉黄丽娟杨柳华力明. N-cadherin对大鼠脊髓损伤后移植神经干细胞存活、增殖与迁移的影响[J]. 基础医学与临床, 2014, 34(3): 372-375.

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