基础医学与临床 ›› 2014, Vol. 34 ›› Issue (12): 1606-1610.

• 研究论文 • 上一篇    下一篇

黄芪减轻血管紧张素II诱导的小鼠血管重塑

裴芳1,王新全2,岳荣川2,黄骥1,黄婕1,王旭开3,于长青4   

  1. 1. 武警重庆总队医院
    2. 第三军医大学 大坪医院 野战外科研究所
    3. 第三军医大学大坪医院野战外科研究所心血管内科,重庆市心血管病研究所
    4. 第三军医大学大坪医院
  • 收稿日期:2014-05-26 修回日期:2014-09-28 出版日期:2014-12-05 发布日期:2014-11-25
  • 通讯作者: 于长青 E-mail:changqingyu-2@163.com
  • 基金资助:
    国家杰出青年科学基金;国家自然科学基金

Astragalus membranaceus alleviates angiotensin II induced vascular remodeling in mice

  • Received:2014-05-26 Revised:2014-09-28 Online:2014-12-05 Published:2014-11-25

摘要: 目的 探讨黄芪对血管紧张素II(AngII)诱导的血管重塑的作用及其可能机制。方法 将小鼠随机分为对照组、AngII组、Ang II+ 氯沙坦 治疗组及Ang II+黄芪治疗组,每组各10只。对照组给予0.9%氯化钠注射液0.2 mL/d灌胃,其余3组均皮下埋微渗透泵,以200 ng/(kg?min)的剂量缓慢释放Ang II建立血管重塑模型。其中氯沙坦治疗组以10 mg/(kg?d)剂量灌胃,黄芪治疗组以20 g/(kg?d)剂量灌胃。所有小鼠总计处理14天。每2天以尾套法测定收缩压。第14 天时处死小鼠,收集主动脉进行HE及Masson染色检测血管重塑情况。RT-PCR检测主动脉I型胶原蛋白转录水平。Western blot检测血管紧张素转换酶2(ACE2)及Ang II 1型受体(AT1R)蛋白表达。结果 黄芪处理可以显著降低Ang II引起的血压升高(P<0.05)、减轻Ang II引起的主动脉管壁增厚和纤维化增加、降低I型胶原蛋白mRNA表达(P<0.05)、上调ACE2蛋白表达(P<0.05)并下调AT1R蛋白表达(P<0.05)。结论 黄芪可减轻Ang II诱导的血管重塑,其机制可能是通过上调ACE2和下调AT1R发挥作用。

关键词: 血管紧张素II, 血管重塑, 纤维化, 黄芪

Abstract: Objective To investigate the effect of astragalus membranaceus on angiotensin II (AngII) induced vascular remodeling in mice and its underlying mechanism. Methods Mice were randomly divided into 4 groups (n=10/group) including control group, AngII group, AngII+Losartan group and AngII+Astragalus group. In control group, mice were given 0.2 ml/d normal saline by gastric gavage. In the other 3 groups, mice were given 200 ng/(kg?min) AngII by an osmotic pump embedded subcutaneously. In addition, in AngII+Losartan group, mice were given 10 mg/(kg?d) Losartan by gastric gavage; in AngII+Astragalus group, mice were given 20 g/(kg?d) by gastric gavage. All the mice were treated for 14 days. Systolic pressure was measured by the tail-cuff method every 2 days. Mice were sacrificed on day 14 and aortas were collected. HE and Masson’s staining were performed to observe vascular remodeling. RT-PCR was carried out to detect transcriptional expression of collagen I. Western Blot was performed to detect expression of angiotensin converting enzyme 2 (ACE2) and angiotensin II type 1 receptor (AT1R). Results Administration of astragalus membranaceus significantly decreased AngII induced blood pressure elevation (P<0.05), attenuated thickening and fibrosis of aorta, decreased mRNA level of collagen I (P<0.05), up-regulated ACE2 expression (P<0.05) and down-regulated AT1R expression (P<0.05). Conclusion Astragalus membranaceus alleviates vascular remodeling induced by AngII in mice, possibly through up-regulating expression of ACE2 and down-regulating AT1R.

Key words: angiotensin II, vascular remodeling, fibrosis, astragalus membranaceus

中图分类号: