基础医学与临床 ›› 2014, Vol. 34 ›› Issue (12): 1601-1605.

• 研究论文 • 上一篇    下一篇

异甘草素增强大鼠脑基底动脉平滑肌细胞

田伟伟1,王蕊1,张传林1,赵磊1,马克涛2,李丽1,司军强1   

  1. 1. 新疆石河子大学医学院生理教研室
    2. 新疆石河子市石河子大学医学院
  • 收稿日期:2014-05-12 修回日期:2014-07-18 出版日期:2014-12-05 发布日期:2014-11-25
  • 通讯作者: 司军强 E-mail:160483868@qq.com
  • 基金资助:
    基于缝隙连接的新疆哈萨克族原发性高血压发病机制研究

Isoliquiritigenin enhanced BKCa-mediated outward currents of basilar artery VSMC in rat

  • Received:2014-05-12 Revised:2014-07-18 Online:2014-12-05 Published:2014-11-25

摘要: 目的 研究异甘草素(ISL)对Wistar大鼠脑基底动脉的影响。方法 大鼠脑基底动脉依次给予10-5 mol/L PE、10-5 mol/L PE+10-4 mol/L ISL和10-5 mol/L PE灌流后,用压力型小动脉仪测量脑基底动脉的直径;用全细胞膜片钳观察10-7、10-6、10-5、10-4和3×10-4mol/L ISL对大鼠脑基底动脉平滑肌细胞(VSMC)电流的影响。结果 1) 10-4 mol/L ISL舒张脑基底动脉( p<0.05);2) ISL呈电压依赖性和浓度依赖性增强脑基底动脉VSMC外向电流( p<0.05),ISL增强脑基底动脉VSMC外向电流的EC50为9.5μmol/L ;给予10-3mol/L四乙胺( TEA,BKCa通道阻断剂)3min后,可抑制ISL对脑基底动脉VSMC外向电流的增强作用,并使外向电流减小到(86±22) pA,只有对照组的0.65±0.04倍( p<0.05)。结论 ISL通过增强BKCa通道介导的外向电流舒张Wistar大鼠脑基底动脉。

关键词: 脑基底动脉, 异甘草素, 大电导钙激活钾通道, 膜片钳

Abstract: Objective To study the effect of ISL on the cerebral basilar artery in Wistar rat. Methods After perfusion with 10-5 mol/L PE、10-5 mol/L PE+10-4 mol/L ISL and 10-5 mol/L PE, pressure myograph was used to measure the diameter of the cerebral basilar artery in rat ; The whole cell patch clamp recording technique was used to investigate the effects of 10-7、10-6、10-5、10-4 and 3×10-4mol/L ISL on the membrane current of VSMC in rat cerebral basilar artery. Results 1) 10-4 mol/L ISL relaxed the cerebral basilar artery(p<0.05). 2) ISL enhanced the membrane current of VSMC in both voltage-dependent and concentration-dependent manners(p<0.05). EC50 of ISL enhanced the VSMC outward current was 9.5μmol/L. In the presence of 10-3 mol/L TEA for 3 min, the enhanced current induced by ISL in VSMCs was ablished, and the current reduced to (86±22) pA, 0.65 ± 0. 04 times compared with the control group( p<0.05). Conclusion ISL relaxed the cerebral basilar atery via the enhancement of the outward current mediated by BKCa channel in Wistar rat.

Key words: cerebral basilar atery, ISL, BKCa channel, patch clamp

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