阿托伐他汀通过转录因子AP1抑制AS大鼠VSMC增殖及促凋亡

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (10): 1339-1343.

阿托伐他汀通过转录因子AP1抑制AS大鼠VSMC增殖及促凋亡

李玉媚¹,王辉²,康敏³,张文宇¹,陈伟¹,胡楠¹,欧和生¹

1. 广西医科大学
2. 广西医科大学药学院
3. 广西医科大学第一附属医院

收稿日期:2014-03-20 修回日期:2014-05-22 出版日期:2014-10-05 发布日期:2014-09-25
通讯作者: 欧和生 E-mail:1297587897@qq.com
基金资助:
家族性高胆固醇血症相关功能突变的组学筛查及基因敲除小鼠模型的建立

Atorvastatin inhibits the proliferation and ptomotes apoptosis of VSMC by the transcription factor AP1 in rats

Received:2014-03-20 Revised:2014-05-22 Online:2014-10-05 Published:2014-09-25

摘要/Abstract

摘要： 目的探讨阿托伐他汀对动脉粥样硬化(AS)大鼠主动脉平滑肌细胞（VSMC）调节及转录因子AP1的作用。方法 80只大鼠随机分为: 普通饲料组、AS组、阿托伐他汀高低剂量组(40和10mg/kg?d)。用原位TUNEL检测细胞凋亡；用免疫组化检测血管中AP1和α-SM-actin蛋白表达；用蛋白免疫印迹法检测血管相关蛋白AP1表达。结果阿托伐他汀高和低剂量组TG、TC和LDL明显下降( P < 0.05)。阿托伐他汀抑制AS大鼠VSMC增殖，促进其促凋亡(P<0.05)。阿托伐他汀干预后AS大鼠VSMC中AP1和α-SM-actin蛋白表达明显减少(P<0.05)。结论阿托伐他汀通过下调AP1抑制AS大鼠VSMC增殖及促凋亡。

关键词: 关键词：阿托伐他汀, 血管平滑肌细胞, AP1, α-SM-actin

Abstract: Objective To investigate atorvastatin inhibits the proliferation and ptomotes apoptosis of VSMC bythe transcription factor AP1 in rats.Methods 80 rats were randomly divided into four groups: normal group,AS group, high-dose atorvastatin group (40mg/kg?d), low-dose atorvastatin group (10mg/kg?d).The VSMC apoptosis was detected by TUNEL assay.The protein expression level of AP1 andα-SM-actin were measured by immunohistochemical analysis and western blotting. Results TG、TC and LDL were significantly decreased in two atorvastatin groups (P<0.05). Atorvastatin significantly inhibited the VSMC proliferation and induced apoptosis (P<0.05). Atorvastatin regulated AP1 andα-SM-actin protein expression was significantly decreased(P<0.05).Conclusions Our results showed that the atorvastatin suppresses proliferation and promote apoptosis of VSMC through down-regulation of AP1 expression in rats.

Key words: Key words:atorvastatin , vascular smooth muscle cell, AP1, α-SM-actin

李玉媚王辉康敏张文宇陈伟胡楠欧和生. 阿托伐他汀通过转录因子AP1抑制AS大鼠VSMC增殖及促凋亡[J]. 基础医学与临床, 2014, 34(10): 1339-1343.

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