丹参酮ⅡA通过诱导C/EBPβ表达促进人源NB4和MR2细胞株分化

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (9): 1123-1128.

丹参酮ⅡA通过诱导C/EBPβ表达促进人源NB4和MR2细胞株分化

张开基¹,王季石¹,李健²,曹金竹³,羊裔明²

1. 贵阳医学院附属医院
2. 四川大学华西医院
3. 贵阳职业护理学院

收稿日期:2012-11-06 修回日期:2012-12-28 出版日期:2013-09-05 发布日期:2013-08-28
通讯作者: 张开基 E-mail:zhangkaijipzh@126.com

TanⅡA triggered the differentiation of NB4 and MR2 cells by up-regulated expression of C/EBPβ

Received:2012-11-06 Revised:2012-12-28 Online:2013-09-05 Published:2013-08-28
Contact: ZHANG KAIJI E-mail:zhangkaijipzh@126.com

摘要/Abstract

摘要： 目的：探索在丹参酮ⅡA（TanⅡA）诱导NB4和MR2细胞分化过程中C/EBPβ（CAAT/enhancer-binding proteinβ）的表达。方法：用TanⅡA干预NB4和MR2细胞120h，通过形态学和膜表面标志检测NB4和MR2细胞的分化情况明确TanⅡA对NB4和MR2细胞的分化效应；0,0.1,1和10mg/L TanⅡA干预NB4和MR2细胞，通过RT-PCR及Western blot测定C/EBPβRNA和蛋白水平表达的变化确定C/EBPβ与TanⅡA浓度的关系；通过膜表面标志检测NB4和MR2细胞的分化情况明确TanⅡA浓度与NB4和MR2细胞分化间的关系。结果：TanⅡA能诱导NB4和MR2细胞分化（p<0.05）；TanⅡA能从RNA和蛋白水平诱导C/EBPβ表达升高并与浓度成正相关（p<0.05）；TanⅡA能通过非剂量依赖的模式诱导NB4和MR2细胞分化，其引起最大分化效果的浓度为1mg/L。结论：TanⅡA能有效促进NB4和MR2细胞分化；TanⅡA通过上调C/EBPβ的表达发挥分化效应；TanⅡA最强作用浓度为1mg/L。

关键词: 丹参酮ⅡA, C/EBPβ, 分化

Abstract: Objective：To Investgiate the expression of C/EBPβ in APL cells during differentiation induced by TanⅡA. Methods TanⅡA treated NB4 and MR2 cells 120h, the characterizations of differentiation were examined by morphology and membrane differentiation antigens；The mRNA and protein expressions of C/EBPβ in different concentration TanⅡA-intervened APL cells were examined by RT-PCR and Western-blot；APL cells were treated with different concentration TanⅡA, the characterizations of differentiation were examined by membrane differentiation antigens. Results TanIIA induced the differentiation of ATRA-sensitive and ATRA-resistant APL cells（p<0.05）; TanIIA dose-dependend upregulated the expression of C/EBPβin mRNA and protein（p<0.05）; TanIIA promoted the differentiation of the NB4 and MR2 cells in a dose-independent manner and exerted its strongest effect at a concentration of 1 mg/L. Conclusion TanIIA promoted the differentiation of ATRA-sensitive and ATRA-resistant APL cells and exerted its strongest effect at a concentration of 1mg/L; C/EBPβ played a key role in TanIIA-induced differentiation.

Key words: TanⅡA, C/EBPβ, differentiation

中图分类号:

R557

张开基王季石李健曹金竹羊裔明. 丹参酮ⅡA通过诱导C/EBPβ表达促进人源NB4和MR2细胞株分化[J]. 基础医学与临床, 2013, 33(9): 1123-1128.

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