腺病毒介导的BMP9过表达抑制人骨肉瘤细胞系143B的增殖与迁移

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (9): 1091-1096.

腺病毒介导的BMP9过表达抑制人骨肉瘤细胞系143B的增殖与迁移

杨丹丹¹,刘晨¹,白慧丽¹,李宝林¹,何方²,张汝益¹,施琼¹

1. 重庆医科大学
2. 重庆医科大学检验医学院

收稿日期:2012-10-29 修回日期:2012-11-28 出版日期:2013-09-05 发布日期:2013-08-28
通讯作者: 施琼 E-mail:anniesq8718@yahoo.com.cn
基金资助:
microRNA-30a和microRNA-30e调控Bub1基因影响小鼠早期胚胎发育的机制研究;microRNA的表观遗传学调控在BMP9诱导MSCs成骨分化中的机制研究

Adenovirus-mediated overexpression of BMP9 inhibits proliferation and migration in human osteosarcoma cell line 143B

Received:2012-10-29 Revised:2012-11-28 Online:2013-09-05 Published:2013-08-28
Contact: Qiong SHI E-mail:anniesq8718@yahoo.com.cn

摘要/Abstract

摘要： 目的探讨骨形态发生蛋白9（BMP9）过表达对人骨肉瘤细胞系143B的生物学行为的影响。方法用RT-PCR和Western blot检测骨肉瘤细胞系中BMP9的内源性表达，用表达BMP9的腺病毒重组体（adBMP9）感染内源表达BMP9相对较低的骨肉瘤细胞系，Transwell法检测细胞侵袭，划痕愈合实验检测细胞迁移，MTT法和结晶紫染色法检测细胞的增殖，，Hoechst 33258染色法检测凋亡，平板集落形成实验检测集落形成能力。结果adBMP9可以明显减弱骨肉瘤细胞系143B的增殖、迁移、侵袭及集落形成能力（P<0.05），并促进细胞凋亡。结论 ad BMP9能抑制人骨肉瘤细胞系143B的增殖和迁移，促进细胞凋亡。

关键词: BMP9，骨肉瘤，生物学效应

Abstract: Objective The aim of this study is to investiage the effect of bone morphogenetic protein 9(BMP9) on the biological behavior of human osteosarcoma cell line 143B.Methods Using RT-PCR and Western blot annlysis to characterize the endogenous expression of BMP9 in the osteosarcoma cell line. Using a recombinant adenovirus expressing BMP9(adBMP9)to infect the osteosarcoma cell line with relatively low endogenous BMP9,then Transwell assay was used to determine cell invasion; Wound-healing assay was used to determine cell migration;MTT assay and crystal violet staining were used to determine the influence of adBMP9 on osteosarcoma cell proliferarion; Hoechst 33258 assay was used to determine cell apoptosis;Clonoy formation assay was used to determine cell clonoy. Results Osteosarcoma cell proliferation、migration、invasion and colony formation were significantly decreased while the cell apoptosis was induced by adBMP9. Conclusion adBMP9 is able not only to inhibit cell proliferation and migration but also induce cell apoptosis in human osteosarcoma cell line 143B.

Key words: BMP9,osteosarcoma,biological effect

中图分类号:

R738.1

杨丹丹刘晨白慧丽李宝林何方张汝益施琼. 腺病毒介导的BMP9过表达抑制人骨肉瘤细胞系143B的增殖与迁移[J]. 基础医学与临床, 2013, 33(9): 1091-1096.

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