基础医学与临床 ›› 2013, Vol. 33 ›› Issue (6): 757-762.

• 研究论文 • 上一篇    下一篇

Wnt2在肝细胞肝癌中的表达及对HepG2细胞增殖的影响

吴堃1,李辉2,朱卓立3,李生伟2   

  1. 1. 重庆医科大学
    2. 重庆医科大学附属第二医院肝胆外科
    3. 重庆医科大学附属第二医院
  • 收稿日期:2013-01-07 修回日期:2013-03-25 出版日期:2013-06-05 发布日期:2013-05-29
  • 通讯作者: 李生伟 E-mail:cqlsw@yahoo.com.cn

Wnt2 expression in Hepatocellular Carcinoma and its inhibitory effect on the proliferation of HepG2

  • Received:2013-01-07 Revised:2013-03-25 Online:2013-06-05 Published:2013-05-29

摘要: 目的 探讨Wnt2在原发性肝细胞肝癌(HCC)中的表达及转染靶向Wnt2的siRNA对人肝癌HepG2细胞的抑制效果。方法 实时荧光定量PCR法和免疫组化法检测Wnt2在肝癌组织,癌旁组织及正常肝组织的表达差异;使用siRNA沉默人肝癌HepG2细胞Wnt2的表达后,检测Wnt2基因和蛋白的表达变化,并检测细胞的增殖和周期变化。结果 1)在上述组织中,Wnt2在癌内表达最高,癌旁次之,正常肝最低。2)使用siRNA沉默人肝癌HePG2细胞Wnt2后,Wnt2基因和蛋白表达下调,细胞增殖受抑制,其生长主要停滞在G0/G1期,而S期细胞所占比例下降。结论 肝细胞肝癌中存在Wnt2的高表达;siRNA可抑制HepG2细胞Wnt2的表达,并抑制HepG2生长,Wnt2基因可能是调控肝癌基因表达的新的靶基因。

关键词: 肝细胞肝癌, Wnt2, RNA干扰

Abstract: Objective To explore the expressions of Wnt2 in human hepatocellular carcinoma(HCC) and the suppressive effect of knocking down Wnt2 expression by RNAi on Hepatocellular cell line HepG2. Methods Real-time quantitative PCR (RT-qPCR) method and immunohistochemistry methods was used to detect the expression of Wnt2 in HCC, paired Paracancerous tissue and normal liver tissue;After RNAi was used to knock down the expression of Wnt2 in HepG2,testing the change of Wnt2 and cell proliferation level and cycle change. Result 1).Wnt2 expressed in the highest in cancer, paired Paracancerous tissue is the second smallest,and normal liver minimum. 2). Using RNAi technology,The expression of Wnt2 was downregulated.HepG2 proliferation was inhibition and the cell growth mainly detained in G0/G1 and the percentage of S decreased. Conclusion Wnt2 was over expression in Hepatocellular Carcinoma;siRNA can inhibit expression of Wnt2 as well as the growth of HepG2.Wnt2 may be a novel target in gene regulation of Hepatocellular Carcinoma.

Key words: hepatocellular carcinoma, Wnt2, RNA interference

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