关键词: 角膜新生血管, 三维培养, microRNA, 靶基因预测

Abstract: Objective To investigate the function and mechanism of miR-184 on cornea neovascularization induced by alkali burning. Methods cornea neovascularization(CRNV) was induced by alkali burning. HUVEC was cultured on Three-dimensional Matrigel. qRT-PCR was performed to analyze microRNA-184 expression in vivo and in vitro. Predicted and analyzed the target genes of microRNA-184 by bioinformatics method. Results Delvelop a model for angiogenesis both in vivo and in vitro. The expression of miR-184 on CRNV (0.145±0.013) was sigificantly less than transparent cornea (1.015±0.189) (P ＜0.01), and was just absolutely miniscule on HUVEC(0.007±0.004 ) (P ＜0.05). The targets of miR-184 predicted was associated with VEGF singaling pathways. Conclusion The marked decrease of miR-184 expression during angiogenesis and target prediction suggest a potential role and mechanism of miR-184 played by VEGF singaling pathways on CRNV.

Key words: cornea neovascularization, three-dimensional culture, microRNA, target gene prediction