关键词: 肿瘤坏死因子, 人生长激素启动子, 垂体腺瘤, 基因治疗

Abstract: Objective To construct a gene therapy system regulated by human growth hormone promoter and evaluate its inhibitory effect on pituitary growth hormone adenoma in vitro. Methods To obtain pSNAV2.0-hGHp-TNFα via enzyme digestion and linker reaction on the basis of pSNAV2.0-hGHp-EGFP and pSNAV2.0-TNFα，then authenticate the targeting ability of hGHp through transfection with Lipofectamine, detect TNFα gene expression with ELISA kit and explore the inhibitory effect of TNFα expression on in vitro pituitary GH adenoma with MTT kit. Results The sequence of pSNAV2.0-hGHp-TNFα is proved correct. Green fluorescence is only detected in GH3 cells but not in control cells after transfection of pSNAV2.0-hGHp-EGFP. The proliferation of GH3 cells transfected with pSNAV2.0-hGHp-TNFα is obviously suppressed compared with control group（P value＜0.05）. Conclusions We successfully construct pSNAV2.0-hGHp-TNFα for the first time and this hGHp regulating gene therapy system can specifically and effectively suppress the proliferation of in vitro pituitary GH adenoma.

Key words: Tumor necrosis factor, Human growth hormone promoter, Pituitary adenoma, Gene therapy