髓样分化因子88与人卵巢癌紫杉醇耐药细胞株A2780/Taxol耐药性相关

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (5): 519-524.

髓样分化因子88与人卵巢癌紫杉醇耐药细胞株A2780/Taxol耐药性相关

邹冬玲¹,李少林²,袁梨¹,周琦¹

1. 重庆医科大学
2. 重庆医科大学放射医学教研室

收稿日期:2013-01-21 修回日期:2013-03-20 出版日期:2013-05-05 发布日期:2013-05-29
通讯作者: 邹冬玲 E-mail:13570049@qq.com

The relationship between MyD88 and Paclitaxel-resistance of A2780/Taxol cell line

1. Chongqing cancer hospital
2. Dept. of Radiation Medicine,Chongqing Medical University
3.

Received:2013-01-21 Revised:2013-03-20 Online:2013-05-05 Published:2013-05-29
Contact: zou dongling E-mail:13570049@qq.com

摘要/Abstract

摘要： 目的本研究希望通过外源改变MyD88的表达，明确其与紫杉醇耐药的相关性，检测MyD88对细胞生物学活性的影响。方法通过在A2780/Taxol细胞中敲低和过表达MyD88后，利用real-time PCR和Western blot分别从mRNA 和蛋白质水平检测MyD88的表达变化；CCK-8法检测细胞转染前后对紫杉醇耐药性的变化，流式技术检测MyD88对细胞周期和凋亡的影响。结果敲低MyD88表达水平，耐药细胞株A2780/Taxol对紫杉醇的耐药性明显降低(p<0.01)，抗凋亡能力减弱(p<0.01)；过表达MyD88后，细胞对紫杉醇耐药性增加(p<0.05)。结论抑制卵巢癌细胞中MyD88的表达, 能促进耐药细胞株对紫杉醇的敏感性，为临床卵巢癌耐药患者以MyD88为靶向的基因治疗提供了新思路和手段。

关键词: 关键词：卵巢癌, 紫杉醇耐药, MyD88

Abstract: Objective The study aimed to make clear the relationship between MyD88 and the resistance of ovarian cancer cell to paclitaxel. Methods After knocking-down and over-expressing MyD88 in A2780/Taxol cell line, qRT-PCR and western blot were performed to test the expression change of MyD88 on mRNA and protein level compared with control group, respectively. CCK-8 assay was executed to detect the change of resistance to paclitaxel in A2780/Taxol cells. Flow cytometry was performed to test the affection to Cell cycle and apoptosis of A2780/Taxol cell line. Results By means of inhibiting MyD88, we observed that resistance of A2780/Taxol to paclitaxel declined obviously, and anti-apoptotic significantly weakened. Inversely, over-expression of MyD88 increased cells resistance to paclitaxel. Conclusion Down-regulation of MyD88 increased sensitivity of ovarian cancer cell to paclitaxel, which provide new ideas and targets of gene therapy for the clinical treatment of ovarian cancer resistant patients.

Key words: Key words: ovarian cancer, taxol-resistant, MyD88

中图分类号:

R 453

邹冬玲李少林袁梨周琦. 髓样分化因子88与人卵巢癌紫杉醇耐药细胞株A2780/Taxol耐药性相关[J]. 基础医学与临床, 2013, 33(5): 519-524.

[1]	孟德权, 秦欣, 陈玲, 覃军. 白术多糖抑制重症肺炎模型大鼠的炎性反应[J]. 基础医学与临床, 2024, 44(9): 1263-1268.
[2]	王琼, 董倩兰, 朱燕亭, 金刚, 张琳萍. 桔梗皂苷D减轻大鼠肾缺血/再灌注损伤[J]. 基础医学与临床, 2023, 43(8): 1222-1228.