关键词: 软骨组织工程, 软骨源性形态发生蛋白1, 骨髓间充质干细胞, 腺病毒转染

Abstract: Objective To explore the clinical application of BMSCs in PLGA scaffold transfected by CDMP and to make a preliminary assessment on the repairing effect. Methods Using the reverse transcriptase polymerase chain reaction ( RT-PCR ) and Western blot for detection of hCDMP1mRNA and protein expression; using immunohistochemistry for detection of collagen type II ( Col II ) as well as glycosaminoglycan ( GAG ) expression. The transfected and untransfected cell scaffold culture systems were implanted into the rabbit thyroid cartilage defects and the culturing systems were analyzed at the gross level as well as at the histological level to observe the ability to repair cartilage defects. Results Adenovirus infection could be successfully transfected into BMSCs of exogenous hCDMP1gene, and obtained the stable expression; as compared with the control group, the BMSCs transfected by hCDMP1 gene enhanced the secretion of type II collagen, GAG and cartilage specific matrix ability; transfected cell scaffold complex could effectively repair laryngeal cartilage defects. Conclusion BMSCs / PLGA 3D scaffold composite transfected by hCDMP1 gene can repair laryngeal cartilage defects more effectively.

Key words: Cartilage tissue engineering, Cartilage-derived morphogenetic protein1, Bone marrow mesenchymal stem cells, Adenovirus infection