基础医学与临床 ›› 2013, Vol. 33 ›› Issue (12): 1576-1580.

• 研究论文 • 上一篇    下一篇

木犀草素预处理减轻H2O2诱导的乳鼠心肌细胞凋亡

印媛君,余美荣,蒋福生,丁志山   

  1. 浙江中医药大学
  • 收稿日期:2013-05-28 修回日期:2013-09-23 出版日期:2013-12-05 发布日期:2013-11-28
  • 通讯作者: 印媛君 E-mail:yinyuanjun1999@126.com
  • 基金资助:
    浙江省教育厅

Luteolin pretreatment mitigate apoptotosis on neonatal rat myocardial cells by hydrogen peroxide induction

  • Received:2013-05-28 Revised:2013-09-23 Online:2013-12-05 Published:2013-11-28
  • Contact: Yuan-Jun YIN E-mail:yinyuanjun1999@126.com

摘要: 目的 探讨木犀草素预处理对H2O2诱导的乳鼠心肌细胞抗凋亡及抗氧化作用。方法 取1-3d乳鼠的心肌细胞,常规培养,分为对照组、模型组、1、10、50、100和200μmol/L浓度的木犀草素预处理组,预处理2h后,用1mmol/LH2O2氧化损伤2h,检测各组MTT值,进而选择100μmol/L浓度的木犀草素作为预处理组。观察不同组别细胞形态学、细胞搏动频率、Hoechest33342-PI双染和Rhodamine 123染色检测MMP,检测培养液中LDH、MDA及SOD活性等。结果 与模型组相比,用木犀草素预处理后的心肌细胞活力良好(P<0.01),形态学及搏动情况良好(P<0.01),细胞凋亡下降,细胞线粒体损伤程度减少(P<0.01),SOD活性提高(P<0.01),LDH及MDA的产生受到抑制(P<0.01)。结论 木犀草素预处理对H2O2诱导的心肌细胞损伤具有一定的保护作用,木犀草素抗凋亡作用可能与其抗氧化作用有关。

关键词: 木犀草素, 心肌细胞, H2O2诱导, 抗凋亡, 抗氧化

Abstract: Objective Anti-apoptotosis and antioxidant effect of luteolin pretreatment on neonatal rat myocardial cells induced by hydrogen peroxide. Methods Myocardial cells are clipped from 1-3 days of neonatal rat. Different groups were set after conventional culture,such as normal groups, the model groups,1μmol/L、10μmol/L、50μmol/L、100μmol/L and 200μmol/L luteolin pretreatment groups. After pretreatment two hours and the 1mmol/L H2O2 oxidative injury 2 hours, MTT datas are detected each group. We selected 100μmol/L luteolin as the pretreatment groups of baseing on the results of the MTT. The different groups are observed about cell morphology, cell pulsating, Hoechst33342-PI double-staining and detected MMP by Rhodamine 123 staining. The activity is detected of LDH,MDA and SOD,and detection of apoptosis . Results Compared with model groups, luteolin pretreatment groups of myocardial cell viability is significantly(P<0.01). The morphology and pulsating are good condition(P<0.01)and apoptosis reduction, and the degree of injury of the mitochondrial is decreased(P<0.01) and SOD activity increased(P<0.01), LDH and MDA generation is suppressed(P<0.01). Conclusion Preconditioning of luteolin on H2O2-induced myocardial injury has a protective effect. Anti-apoptotic effect of luteolin may be related to the antioxidant.

Key words: luteolin, myocardial cell, hydrogen peroxide induced, Anti-apoptotic, antioxidant.

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