基础医学与临床 ›› 2013, Vol. 33 ›› Issue (1): 19-13.

• 最新分子细胞生物学技术专题 • 上一篇    下一篇

环介导等温扩增技术及其在病原微生物检测中的应用

肖维威,周琳华,郑文岭,马文丽   

  1. 南方医科大学基因工程研究所
  • 收稿日期:2012-11-02 修回日期:2012-11-19 出版日期:2013-01-05 发布日期:2012-12-25
  • 通讯作者: 马文丽 E-mail:wenli668@gmail.com

Loop-mediated isothermal amplification technology and its application in pathogenic microorganism detection

  • Received:2012-11-02 Revised:2012-11-19 Online:2013-01-05 Published:2012-12-25

摘要: 环介导等温扩增(LAMP)技术是近年发展起来的一项新的快速核酸恒温扩增技术,具有高效、快速、特异、易检测、易操作等特点,自面世以来被科学家认为是能替代常规PCR的一项扩增技术,非常适用于现场检测和基层检测。本文就LAMP技术的原理、特点、进展及其在病原微生物检测中的应用进行了简要的概述。

Abstract: Loop-mediated isothermal amplification (LAMP) technology is a novel rapid nucleic acid isothermal amplification technology developed in recent years. Because of its high effiency, rapidity, specificity, and simplicity, scientists believe it will be a common amplification technology replacing the conventional PCR method. This paper briefly reviews the principle of the technology, techinical characteristics, progress, and its application in pathogenic microorganism detection.