5-FU诱导人肝细胞癌MDR的比较蛋白质组学研究

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (9): 1015-1020.

5-FU诱导人肝细胞癌MDR的比较蛋白质组学研究

韦艳¹,张海英²,梁钢²

1. 武警广西总队医院药局
2. 广西医科大学

收稿日期:2011-10-20 修回日期:2011-12-27 出版日期:2012-09-05 发布日期:2012-08-28
通讯作者: 韦艳 E-mail:dfn0201@hotmail.com
基金资助:
骨髓间充质干细胞瘤化中STAT3信号通路过度激活的作用机制与规避研究;广西地方性高发疾病防治研究重点实验室基金

Comparative proteomics analysis of human hepatocellular carcinoma MDR induced by 5-FU

Received:2011-10-20 Revised:2011-12-27 Online:2012-09-05 Published:2012-08-28

摘要/Abstract

摘要： 摘要：目的寻找与人肝细胞癌多药耐药相关的蛋白质分子，为进一步研究人肝细胞癌多药耐药机制提供依据。方法采用体外培养人肝癌细胞BEL-7402及其耐5-氟尿嘧啶（5-FU)细胞Bel-FU，MTT法检测Bel-FU的多药耐药性,采用双向凝胶电泳技术分析细胞BEL-7402与Bel-FU之间的差异表达蛋白，经MALDI-TOF/ TOF 质谱鉴定。Western-blot验证2-DE及质谱的检测结果。结果与Bel-7402比较, Bel-FU中有24个蛋白表达上调，其中包括FAK、TM3、ORP150、CRT、NSE 、80K-H protein、EF-1-D、phosphoprotein等，12个蛋白表达下调。Western-blot法检测到蛋白FAK、CRT在Bel-FU中高表达，与2-DE结果一致。结论通过建立人肝癌细胞BEL-7402及其耐5-氟尿嘧啶细胞Bel-FU的2-DE图谱筛选了多药耐药相关的差异蛋白，为人肝细胞癌MDR的分子机制研究奠定了基础。

关键词: 关键词：肝细胞癌, 多药耐药, 比较蛋白质组学, 双向凝胶电泳

Abstract: ABSTRACT Objective To search the protein as to human hepatocellular carcinoma MDR and provide evidence for the mechanism study of hepatocellular carcinoma MDR.Methods Human hepatocellular carcinoma cell line Bel-7402 and 5-FU resistant cell line Bel-FU were cultured in vitro.The MDR of Bel-FU was detected by MTT assay,the screen the differential expression protein between Bel-7402 and Bel-FU by 2-DE and MALDI-TOF/ TOF, then Western-blot checked the result . Results compared with those in Bel-7402, 24 proteins were up-regulated in Bel-FU including FAK、TM3、ORP150、CRT、NSE 、80K-H protein、EF-1-D、phosphoprotein and so on ,and 12 proteins were down-regulated. Western blot confirmed that FAK、CRT were up-regulated in Bel-FU, they were in accordance with the results of 2- DE.Conclusion The differential expression protein between Bel-7402 and Bel-FU pay a way for the study of molecule mechanism as to MDR.

Key words: Key words: hepatocellular carcinoma, Multidrug Resistance, Comparative proteomics, 2-DE

韦艳张海英梁钢. 5-FU诱导人肝细胞癌MDR的比较蛋白质组学研究[J]. 基础医学与临床, 2012, 32(9): 1015-1020.

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