基础医学与临床 ›› 2012, Vol. 32 ›› Issue (7): 814-818.

• 研究论文 • 上一篇    下一篇

维甲酸诱导的小鼠神经管畸形模型中神经系统相关基因的表达情况分析

周斌1,张靖2,潘艳芳1,阴彬3,彭小忠4   

  1. 1. 中国医学科学院
    2. 中国医学科学院基础医学研究所&北京协和医学院 基础学院 生物化学与分子生物学
    3. 中国医学科学院基础医学研究所 北京协和医学院基础学院
    4. 中国医学科学院 基础医学研究所 北京协和医学院 基础学院 医学分子生物学国家重点实验室
  • 收稿日期:2012-04-17 修回日期:2012-05-24 出版日期:2012-07-05 发布日期:2012-06-20
  • 通讯作者: 彭小忠 E-mail:pengxiaozhong@pumc.edu.cn

Expression of nervous system related gene in retinoic acid –induced neural tube defects of mice

  • Received:2012-04-17 Revised:2012-05-24 Online:2012-07-05 Published:2012-06-20
  • Contact: Xiao-zhong PENG E-mail:pengxiaozhong@pumc.edu.cn

摘要: 摘要: 目的: 研究在全反式维甲酸诱导小鼠神经管畸形模型中神经系统相关基因(ASH2L,HDAC4,NSPC1与DOK5)的表达变化。方法: 取8只E8.5的C57/BL6孕鼠随机分为两组:对照组(4只),模型组(4只)。对照组腹腔注射橄榄油,模型组腹腔注射全反式维甲酸;E13.5取胚胎。采用Real-time PCR检测两组小鼠脑和脊髓中ASH2L,HDAC4,NSPC1与DOK5的mRNA表达;采用Western Blotting技术检测两组小鼠脑和脊髓中ASH2L,HDAC4,NSPC1与DOK5的蛋白表达情况。结果:全反式维甲酸可诱导小鼠出现典型神经管畸形;与对照组相比,全反式维甲酸处理组的致畸胚胎脑和脊髓中,ASH2L HDAC4, NSPC1和DOK5的mRNA表达有所下降(P<0.05);同时这些基因的蛋白水平也显著下降 (P<0.05)。结论:ASH2L,HDAC4,NSPC1和DOK5可能是抑制全反式维甲酸致神经管畸形的潜在基因。

关键词: 关键词:全反式维甲酸, ASH2L,HDAC4,NSPC1与DOK5, 神经管畸形

Abstract: Abstract: Objective To study the expression of four nervous system related genes: ASH2L, HDAC4, NSPC1and DOK5 in the all trans retinoic acid -induced mouse neural tube defect model. Methods: Divide eight E8.5 C57/BL6 pregnant mice randomly into two groups: control group and experimental group . Control group, intraperitoneal injection of olive oil, the experimental group by intraperitoneal injection of all-trans retinoic acid; E13.5 take embryos. Real-time PCR were detected in mouse brain and spinal cord of ASH2L, HDAC4, NSPC1 and DOK5 mRNA expression; Western Blotting detected two groups of mice brain and spinal cord ASH2L, HDAC4, NSPC1 and DOK5 of protein expression.Results All-trans retinoic acid can induce a typical neural tube defects in mice. Compared with the control group, the expression of mRNA declined in fetal mice brain and spinal cord which is treated with all trans RA (P <0.05); Meanwhile, the protein levels of these genes was also significantly decreased (P <0.05). Conclusion ASH2L, HDAC4, NSPC1 and DOK5 may be the may underlying genes of inhibiting all trans RA-induced neural tube defects.

Key words: Key words: all trans retinoic acid, ASH2L, HDAC4, NSPC1, DOK, Neural tube defects.

中图分类号: