关键词: PANDER, 胰岛β细胞, 胰岛素抵抗

Abstract: Objective To analyze PANDER expression in pancreatic islets of type 1 and type 2 diabetic mice, and probe the correlationship of PANDER expression and insulin resistance in type 2 diabetic mice. Methods PANDER mRNA and protein levels were analyzed in the pancreas of STZ-induced type 1 diabetic mice, Akita diabetic mice, type 2 diabetic db/db mice. Result In the pancreas of STZ-induced and Akita diabetic mice, PANDER mRNA (STZ：1.00±0.17 vs. 0.25±0.06，P<0.01；Akita：1.00±0.19 vs.0.17±0.04，P<0.001) and protein (STZ：1.00±0.18 vs. 0.16±0.11) levels were significantly decreased when compared with non-diabetic control mice. In contrast, both PANDER mRNA (1.00±0.05 vs. 2.13±0.46，P<0.05) and protein (1.00±0.28 vs. 3.58±0.36，P<0.01) levels were increased in the pancreas of db/db mice as compared with db/m mice. Oral treatment of db/db mice for 1 month with rosiglitazone reduced pancreatic PANDER mRNA(1.00±0.13 vs. 0.28±0.06，P<0.01) and protein expression in pancreatic islets of db/db mice, with improved insulin resistance, hyperinsulinemia and hyperglycemia. Conclusions PANDER expression was reduced in the pancreas of type 1 diabetic mice, whereas its expression was increased in the pancreas of type 2 diabetic mice.

Key words: PANDER, islet beta cells, insulin resistance