2009甲型H1N1疫苗株与普通季节性H1N1疫苗株在呼吸道细胞中感染效率的比较

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (6): 613-617.

2009甲型H1N1疫苗株与普通季节性H1N1疫苗株在呼吸道细胞中感染效率的比较

王玉国¹,洪晓栩²,何丽芳³,杨宁¹,王丽丽³,赵琼英³,蒋澄宇⁴

1. 中国医学科学院&北京协和医学院
2. 国家食品药品监督管理局
3. 云南沃森生物技术股份有限公司
4. 中国医学科学院基础医学研究所北京协和医学院基础学院

收稿日期:2012-04-05 修回日期:2012-04-25 出版日期:2012-06-05 发布日期:2012-05-25
通讯作者: 蒋澄宇 E-mail:chengyujiang@gmail.com
基金资助:
科技部973项目;传染病防治科技重大专项

Comparation of infection efficiency of H1N1 virus in human airway epithelial cells

Received:2012-04-05 Revised:2012-04-25 Online:2012-06-05 Published:2012-05-25
Contact: Cheng-yu JIANG E-mail:chengyujiang@gmail.com

摘要/Abstract

摘要： 摘要：目的探讨2009甲型H1N1疫苗株与季节性H1N1疫苗株在人呼吸道细胞中感染效率的差异。方法用间接免疫荧光法检测H1N1病毒感染A549、CNE-2Z、H1650、Hep-2和MRC-5细胞的效率；用间接免疫荧光法检测抑制因子对H1N1病毒侵入细胞的影响。结果（1）2009甲型H1N1疫苗株感染人呼吸道细胞的效率：H1650>A549>CNE-2Z>MRC-5>Hep-2；季节性H1N1疫苗株感染人呼吸道细胞的效率A549>CNE-2Z>H1650>MRC-5>Hep-2；（2）2009甲型H1N1疫苗株和季节性H1N1疫苗株均依赖于内吞作用进入细胞。结论在A549细胞中，季节性H1N1疫苗株的感染效率明显高于甲型H1N1疫苗株；在H1650细胞中，甲型H1N1疫苗株的感染效率明显高于季节性H1N1疫苗株。

关键词: 关键词：甲型H1N1病毒, 流感病毒, 呼吸道细胞, 感染效率

Abstract: Abstract: Objective Define the infection efficiency of 2009 novel swine-origin influenza A virus and seasonal influenza virus in human airway epithelial cells. Methods Infection efficiency of H1N1 virus in A549, CNE-2Z, H1650, Hep-2 and MRC-5 was examined by indirect immunofluorescence assay. Effect of inhibitors in human airway epithelial cells infected by H1N1 virus was examined by indirect immunofluorescence assay. Results （1）Infection efficiency of swine-origin H1N1 virus: H1650>A549>CNE-2Z>MRC-5>Hep-2；Infection efficiency of seasonal H1N1 virus: A549>CNE2Z>H1650>MRC-5>Hep-2;（2）Swine-origin H1N1 and seasonal H1N1 both entered into airway epithelial cells by the way of endocytosis. Conclusion Infection efficiency of seasonal H1N1 virus was clearly higher than swine-origin H1N1 virus in A549 cells; Infection efficiency of swine-origin H1N1 virus was clearly higher than seasonal H1N1 virus in H1650 cells as infection time increased.

Key words: Key words: S-OIV H1N1, influenza virus, airway epithelial cells, infection efficiency

中图分类号:

R 373.1

王玉国洪晓栩何丽芳杨宁王丽丽赵琼英蒋澄宇. 2009甲型H1N1疫苗株与普通季节性H1N1疫苗株在呼吸道细胞中感染效率的比较[J]. 基础医学与临床, 2012, 32(6): 613-617.

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