内脏高敏感大鼠结肠Cajal间质细胞c-kit表达增加

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (5): 566-569.

内脏高敏感大鼠结肠Cajal间质细胞c-kit表达增加

丁瑞峰¹,王爱鱼²,²,郭元虎²,²,王宏杰²,²,赵鹏程²,²

1. 包头医学院第一附属医院
2.

收稿日期:2010-12-06 修回日期:2011-11-14 出版日期:2012-05-05 发布日期:2012-04-16
通讯作者: 丁瑞峰 E-mail:rfding721115@163.com
基金资助:
包头市科技计划项目卫生基金

Increased Expression of c-kit in ICC in Colon of Rat with Visceral Hypersensitivity

Received:2010-12-06 Revised:2011-11-14 Online:2012-05-05 Published:2012-04-16

摘要/Abstract

摘要： 摘要：目的检测内脏高敏感大鼠结肠Cajal间质细胞（ICC）c-kit表达，探讨ICC在内脏高敏感中的作用。方法取出生后8~21d的Wistar大鼠，实验组每天直肠注射0.6%的冰醋酸0.3~0.5mL，对照组给予同样剂量的生理盐水。8周龄时行直肠扩张评估内脏敏感性，记录腹部回缩反射(AWR)为3分时的注水量。取降结肠组织，行c-kit免疫组化和Western blot检测。结果与对照组相比，实验组大鼠AWR为3分时的注水量明显减少（P＜0.05）；C-kit阳性细胞数在实验组大鼠结肠中为（23.37±1.88）较对照组（15.33±1.57）明显增加（P＜0.05）；C-kit蛋白表达在实验组（134.5±47.5）较对照组（40.6±30.5）明显增强（P＜0.01）。结论 ICC增多可能是内脏高敏感的发病机制之一。

关键词: 内脏高敏感, 结肠, Cajal间质细胞, 原癌基因蛋白质c-kit

Abstract: Abstract: Objective Through research of the expression of c-kit in interstitial cells of cajal(ICC) in colon of rat with visceral hypersensitivity, to investigate the role of ICC in the pathophysiology of visceral hypersensitivity.Methods 8 to 21 days after birth, the Wistar rats of the experimental group were injected with daily recta glacial acetic acid 0.6% 0.3~0.5ml, the control group were injected the same dose of saline. The visceral sensitivity of 8-week-old rats was evaluated by colorectal distention，and recorded the water injection rate when the score of abdominal withdrawal reflex (AWR) was 3.The distal descending colon was taken to detect for c-kit immunohistochemisty and Western blot.Result Compared with the control group, the water injection rate of the experimental group showed a significant decrease when the score of AWR was 3(P＜0.05);C-kit masculine cells counting in colon in the experimental group significantly increased(P＜0.05);The expression of c-kit protein in colon in the experimental group significantly increased(P＜0.01).Conclusion The increase of ICC of rat with visceral hypersensitivity may be a possible cause of visceral hypersensitivity.

Key words: visceral hypersensitivity, colon, interstitial cells of cajal, proto-oncogene protein c-kit

中图分类号:

R-332

丁瑞峰王爱鱼郭元虎王宏杰赵鹏程. 内脏高敏感大鼠结肠Cajal间质细胞c-kit表达增加[J]. 基础医学与临床, 2012, 32(5): 566-569.

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