基础医学与临床 ›› 2012, Vol. 32 ›› Issue (5): 510-515.

• 研究论文 • 上一篇    下一篇

MiR-21通过抑制RECK表达促进了胆管癌细胞QBC939的侵袭

刘长征1,何小东2,于岚3,刘卫3,陈松森1   

  1. 1. 中国医学科学院基础医学研究所
    2. 北京协和医院
    3. 中国医学科学院北京协和医院
  • 收稿日期:2012-02-20 修回日期:2012-03-22 出版日期:2012-05-05 发布日期:2012-04-16
  • 通讯作者: 刘长征 E-mail:cz-liu@ibms.pumc.edu.cn
  • 基金资助:
    重塑FoxA2-miR-29调控网络可能恢复恢复糖尿病小鼠血糖的稳定调节

MiR-21 modulates the invasiveness of QBC939 cells through negatively regulating RECK expression

  • Received:2012-02-20 Revised:2012-03-22 Online:2012-05-05 Published:2012-04-16
  • Contact: Chang-Zheng LIU E-mail:cz-liu@ibms.pumc.edu.cn

摘要: 目的 研究miR-21在胆管癌组织及细胞系中的表达特征及其在胆管癌发生过程中的功能。方法 利用Real-Time PCR与Northern Blot方法分别分析miR-21在胆管癌组织及胆管癌细胞系QBC939中的表达水平;选择特异抑制剂Anti-miR-21 敲低QBC939细胞内源性miR-21的表达,研究其对细胞增殖及凋亡表型的影响;利用萤光素酶双报告基因以及流式细胞仪分析方法筛选并鉴定miR-21的靶基因;根据靶基因的功能研究miR-21对胆管癌细胞系QBC939体外侵袭能力的影响。结果 表达分析显示, miR-21在胆管癌组织及胆管癌细胞系QBC939中表达均明显上调;细胞表型分析显示,QBC939转染Anti-miR-21后,细胞增殖被抑制,同时凋亡细胞增加明显;靶基因分析显示,miR-21可以抑制RECK的表达,并可以通过两者之间的相互作用,参与QBC939细胞系的体外侵袭调控。结论 miR-21在胆管癌组织及细胞系中表达上调,促进了胆管癌细胞增殖并抑制细胞凋亡;RECK是miR-21在胆管癌细胞中的靶基因,通过抑制其表达,miR-21增强了胆管癌细胞的侵袭能力。

关键词: miR-21, 胆管癌, RECK, 侵袭

Abstract: Objective To investigate the expression profile of miR-21 in human cholangiocarcinoma tissues and QBC939 cell line and probe the function of miR-21 in cholangiocarcinogenesis. Methods MiR-21 expression in human cholangiocarcinoma tissues and QBC939 cell line is measured by using Real-Time PCR and Northern Blot, respectively. Cell growth and apoptosis was analyzed in QBC939 after transfected with Anti-miR-21. Specific target analysis is performed by using dual-reporter gene assay and FACS. In Vitro invasion assay is performed to probe the effect of miR-21 on QBC939 invasiveness. Results Expression analysis reveals that miR-21 levels depicted a significant up-regulation as compared to the matched normal bile duct and miR-21 levels are augmented approximately 3.4-fold in QBC939 cells. Silencing of miR-21 in QBC939 by using anti-miR-21 decreases cell growth and induces cell apoptosis. RECK is identified as a direct effector of miR-21 and miR-21 promotes QBC939 cell invasion in vitro through negatively regulating miR-21-RECK program. Conclusion Increased miR-21 expression is a frequent event in human cholangiocarcinoma. Augmented miR-21 promotes cell growth and dampens cell apoptosis. The invasiveness is enhanced by miR-21 through inhibiting RECK expression.

Key words: miR-21, Cholangiocarcinoma, RECK, Invasion

中图分类号: