维吾尔族妇女宫颈癌早期与中晚期患者血浆蛋白质组的二维液相色谱比较分析

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (4): 417-422.

维吾尔族妇女宫颈癌早期与中晚期患者血浆蛋白质组的二维液相色谱比较分析

纳菲沙.卡德尔¹,郭霞²,武贵臻²,夏米西努尔?伊力克刘开江⁴,阿布力孜.阿布杜拉²

收稿日期:2011-03-22 修回日期:2011-10-19 出版日期:2012-04-05 发布日期:2012-03-21
通讯作者: 阿布力孜.阿布杜拉 E-mail:abulizi_de@126.com
基金资助:
自治区自然科学基金;新疆维吾尔自治区高技术研究发展计划

Comparative analysis of plasma proteome of early and advanced stage of cervical carcinoma of Uighur women by two-dimensional liquid phase chromatography

Received:2011-03-22 Revised:2011-10-19 Online:2012-04-05 Published:2012-03-21

摘要/Abstract

摘要： 目的运用蛋白质二维液相色谱技术，分析维吾尔族妇女宫颈鳞癌患者血浆低丰度蛋白质组谱特征，寻找肿瘤临床早期和中晚期之间的血浆蛋白组差异。方法收集维吾尔族妇女宫颈鳞癌患者血浆标本共46例，其中临床分期为Ⅰ-Ⅱa的早期患者26例，Ⅱb-Ⅳ的中晚期患者20例；利用高丰度蛋白去除方法，制备血浆低丰度蛋白质组样品，经蛋白质二维液相色谱系统（ProteomeLabTM PF-2D）分析，研究不同宫颈癌临床分期患者血浆蛋白质组差异。结果建立了早期和中晚期宫颈鳞癌患者的蛋白质组差异图谱；以2倍以上的血浆蛋白质含量变化作为蛋白质峰组分的定量差异标准，并将宫颈癌早期患者设为对照，综合分析二组的有效蛋白组分，发现中晚期肿瘤患者血浆中5个低丰度蛋白组分含量明显上升，而10个组分下降。结论维吾尔族妇女宫颈鳞癌患者不同临床分期之间存在血浆低丰度蛋白质组差异，为进一步鉴定肿瘤演进过程特异的血浆标志蛋白提供了客观依据。

关键词: 维吾尔族妇女, 宫颈癌, 蛋白质组, 二维液相色谱

Abstract: Objective To analyze the proteomic profile of low abundant plasma proteins in cervical squamous cell carcinoma（CSSC）of Uighur women by using two dimensional liquid phase chromatography, to identify differences of plasma proteome between early and advanced stages of the cancer. Methods Forty-six cases of plasma samples from Uighur women with CSSC were collected，including 26 cases of early stage cancer atⅠ-Ⅱa and 20 cases of advanced stage cancer at Ⅱb-Ⅳ; After preparation of the proteome of low abundant plasma proteins by depletion of high abundant proteins, the proteomic fingerprint features of patients with early stage and advanced stage of cancer were studied by two-dimensional liquid phase chromatography (ProteomeLabTM PF- 2 D) , to establish a distinguishing model of plasma proteome for patients withdifferent stages of cancer. Results A plasma proteome fingerprint model specific to patients with early stage and advanced stage of cancer was established. By setting up the protein content difference of more than two times as a standard for quantitative differences, 5 peaks with ascending and 10 with descending protein content in patients with advanced stage of cancer compared to early stages were found. Conclusion The remarkable difference in low abundant plasma proteomes between early and advanced stages of CSCC in Uighur women may provide an important evidence for the identification of plasma protein markers specific to the cancer progression in the future.

Key words: Uighur women, cervical carcinoma, proteome, 2-D liquid phase chromatography

纳菲沙.卡德尔郭霞武贵臻夏米西努尔?伊力克刘开江阿布力孜.阿布杜拉. 维吾尔族妇女宫颈癌早期与中晚期患者血浆蛋白质组的二维液相色谱比较分析[J]. 基础医学与临床, 2012, 32(4): 417-422.

[1]	脱勋元王琰党云脱淑梅蔡炳昕龙丽霞. 过表达TGF-β1可抑制人宫颈癌细胞系C-33A增殖和促进凋亡[J]. , 2019, 39(12): 1704-1708.
[2]	邹丽莉李文婷王丹琪王曌孙伟. 免疫去除血清高丰度蛋白对组织蛋白质组鉴定的影响[J]. 基础医学与临床, 2015, 35(4): 439-443.
[3]	王志敏包珊. 肝细胞生长因子促进HeLa细胞上皮间质转化[J]. 基础医学与临床, 2015, 35(4): 485-490.
[4]	徐思慧曹保利. 治疗性HPV DNA疫苗的研究进展[J]. 基础医学与临床, 2015, 35(3): 421-424.
[5]	左强强郑威楠张金莉李玉华刘引引龙海晨潘泽民. IFITM1 基因启动子甲基化与新疆维吾尔族妇女子宫颈癌的相关性[J]. 基础医学与临床, 2014, 34(1): 58-61.
[6]	杨佳欣郎景和沈铿曹冬焱荣春红. 宫颈癌血清相关蛋白的初步探讨及鉴定 [J]. 基础医学与临床, 2012, 32(2): 191-194.
[7]	雷晓波孙振民刘新蕾任丽丽王健伟. 肠道病毒71型3C蛋白表达后细胞蛋白质组差异分析[J]. 基础医学与临床, 2011, 31(6): 652-655.
[8]	甄永占;林雅军;骆广玲;赵毓芳;高俊玲. 赖氨大黄酸通过激活JNK诱导宫颈癌HeLa细胞凋亡[J]. 基础医学与临床, 2011, 31(1): 68-72.
[9]	时艳梅肖长义叶红王雅琴吴江锋. 临床宫颈组织标本HPV16 L1蛋白检测[J]. 基础医学与临床, 2010, 30(8): 807-810.
[10]	李晶华王伟娟高婉丽郭蕾冯力民. HPV18 E6 siRNA对宫颈癌细胞增殖及化疗药物敏感性的影响[J]. 基础医学与临床, 2010, 30(5): 500-504.
[11]	封素娟刘旭张彩艳卜祥宁张楠孙媛郭菲李俊发. 低氧预适应小鼠脑皮层内与nPKC 相互作用蛋白鉴定[J]. 基础医学与临床, 2010, 30(3): 225-231.
[12]	吴维光陈亚琼曹秀琴. RNA干扰沉默HIF-1α基因对宫颈癌HeLa细胞定植和侵袭的影响[J]. 基础医学与临床, 2010, 30(3): 284-288.
[13]	任绪义周敏兰张欣宗刘洋虞闰六. 特发性男性不育患者精浆蛋白质分析[J]. 基础医学与临床, 2009, 29(7): 688-690.
[14]	郑智国夏婷李永哲高赟牟瀚舟许沈华许洋. WCX纳米磁珠在宫颈鳞癌血清蛋白质组中的应用及其临床意义[J]. 基础医学与临床, 2009, 29(3): 287-291.
[15]	张彩艳封素娟刘旭卜祥宁张楠郑雅欣袁晓文李晓光李俊发. 脑衰蛋白反应调节蛋白-2参与低氧预适应减轻小鼠脑缺血损伤[J]. 基础医学与临床, 2009, 29(11): 1133-1138.