miR-424负向调节人脂肪源间充质干细胞向成脂细胞的分化

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (2): 114-118.

miR-424负向调节人脂肪源间充质干细胞向成脂细胞的分化

黄姗¹,王世华¹,韩钦²,赵春华³

1. 中国医学科学院，北京协和医学院，基础所组织工程研究中心
2. 中国医学科学院基础医学研究所组织工程中心
3. 中国医学科学院基础研究所组织工程中心

收稿日期:2011-12-14 修回日期:2012-01-12 出版日期:2012-02-05 发布日期:2012-01-12
通讯作者: 赵春华 E-mail:chunhuaz@public.tpt.tj.cn.com

MiR-424 negatively regulates adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells

1.
2. Center of Excellence in Tissue Engineering, Institute of Basic Medical Sciences, CAMS & PUMC
3. Center for Tissue Engineering, Institute of Basic Medical Science,CAMS & PUMC
4. Tissue Engineering Center, CAMS

Received:2011-12-14 Revised:2012-01-12 Online:2012-02-05 Published:2012-01-12

摘要/Abstract

摘要： 目的研究miR-424对人脂肪源间充质干细胞（hAMSCs）向成脂分化的影响。方法用real-time PCR检测hAMSCs成脂分化前后细胞内miR-424水平的变化。在脂质体介导下，用人工合成的miR-424模拟物转染hAMSCs提高细胞内miR-424的含量，随后对其进行成脂诱导。用油红O染色法观察细胞内脂滴形成情况，用Real-Time PCR和Western blot检测成脂关键转录因子PPARγ及相关标记物mRNA的表达。结果 hAMSCs成脂分化后，miR-424表达下调。与对照组细胞相比，转染miR-424模拟物的实验组细胞中miR-424含量明显升高。成脂诱导第8天油红O染色结果显示，实验组脂滴形成显著少于对照组。PPARγ和成脂相关标记物的表达量也出现下调。结论 miR-424可负向调节hAMSCs向脂肪细胞分化。

关键词: miR-424, 人脂肪源间充质干细胞, 成脂分化

Abstract: Object To investigate the effect of miR-424 on adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAMSCs). Methods Expression level of miR-424 during adipogenic differentiation of hAMSCs was detected by Real-Time PCR. hAMSCs were transfected with synthetic miR-424 mimics using siPORT NeoFX Transfection Agent to increase the level of miR-424 in hAMSCs, and then cultured in adipogenic differentiation medium. Oil red O staining was used to observe the accumulation of lipid droplets in hAMSCs. Expression levels of key adipogenic transcription factor PPARγ and adipogenic-related genes were detected by Real-Time PCR and Western blot. Results The expression of miR-424 decreased after hAMSCs were induced to differentiate into adipocytes. Compared to control group, the level of miR-424 was significantly increased in miR-424-mimics-transfection group. Besides，decreasing of lipid droplets accumulation was observed by Oil red O staining on Day 8 of adipogenic differentiation. Moreover, the levels of PPARγ and adipogenic-related markers were also down-regulated in the miR-424-mimics transfection group. Conclusion miR-424 could negatively regulate adipogenic differentiation of hAMSCs.

Key words: miR-424, hAMSCs, adipogenic differentiation

黄姗王世华韩钦赵春华. miR-424负向调节人脂肪源间充质干细胞向成脂细胞的分化[J]. 基础医学与临床, 2012, 32(2): 114-118.