二氯化钴致人结肠癌细胞系CacyBP/SIP核转位

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (11): 1312-1317.

二氯化钴致人结肠癌细胞系CacyBP/SIP核转位

谢福利¹,仇长青²,³,赵盈盈²,³,杨博²,³,冯珊珊²,³,翟惠虹⁴

1. 宁夏师范学院医学院
2. 宁夏医科大学临床学院总医院
3.
4. 宁夏医科大学临床学院总医院消化内科

收稿日期:2012-01-17 修回日期:2012-03-28 出版日期:2012-11-05 发布日期:2012-10-19
通讯作者: 谢福利 E-mail:xfl1971@126.com
基金资助:
CacyBP/SIP在结肠癌细胞中核转位的机制研究

Effect of CoCl2 on CacyBP/SIP nuclear translocation of cell lines of colon carcinoma

Received:2012-01-17 Revised:2012-03-28 Online:2012-11-05 Published:2012-10-19

摘要/Abstract

摘要： 目的观察和探讨低氧可否引起人结肠癌细胞株CacyBP/SIP核转位。方法用人结肠癌细胞株HCT-116的cDNA序列，用分子克隆法构建过表达CacyBP/SIP的绿荧光慢病毒载体，转染至结肠癌SW480细胞，用CoCl2作为刺激因子，激光共聚焦显微镜和Western blot检测CacyBP/SIP的表达及定位。结果构建了过表达CacyBP/SIP的慢病毒载体，转染至结肠癌细胞，CoCl2刺激前CacyBP/SIP定位和表达主要在细胞胞质，刺激后CacyBP/SIP定位和表达在细胞胞质和胞核。结论 CoCl2刺激后可致CacyBP/SIP核转位。

关键词: CacyBP/SIP, CoCl2, 核转位, 结肠癌

Abstract: Objective The thesis aims to observe and evaluate the effect of hypoxia on CacyBP/SIP nuclear translocation of the cell lines of human colon carcinoma. Methods taking cDNA sequence of colon carcinoma cell line HCT-116 cells as the subject, the study mainly builds overexpressed lentiviral vector with Green fluorescent protein CacyBP/SIP by Molecular cloning, and then transfects it into SW480 cells of colon carcinoma. Taking CoCl2 as the Stimulating factor, it tests the location and expression of the CacyBP/SIP with Laser Scanning Confocal Microscope and Western blot. Results the construction of lentiviral vectors for the overexpression of CacyBP / SIP, was transfected into colon cancer cells successfully, and it has been found that before the stimulation of CoCl2, CacyBP/SIP is located and expressed in the cytoplasm, and both in cytoplasm and nucleus after the stimulation of CoCl2. Conclusion The stimulation of CoCl2 can lead to the nuclear translocation of CacyBP/SIP.

Key words: CacyBP/SIP, CoCl2, nuclear translocation, colon cancer

中图分类号:

R574.62

谢福利仇长青赵盈盈杨博冯珊珊翟惠虹. 二氯化钴致人结肠癌细胞系CacyBP/SIP核转位[J]. 基础医学与临床, 2012, 32(11): 1312-1317.

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