基础医学与临床 ›› 2011, Vol. 31 ›› Issue (9): 984-987.

• 研究论文 • 上一篇    下一篇

p38MAPK通过上调组蛋白乙酰化水平影响APP表达

任乐荣1,李良2,郭学文2   

  1. 1. 首都医科大学病理教研室
    2. 首都医科大学
  • 收稿日期:2010-07-19 修回日期:2010-10-14 出版日期:2011-09-05 发布日期:2011-09-05
  • 通讯作者: 李良 E-mail:liliang@ccmu.edu.cn
  • 基金资助:
    市自然科学基金

Effect on APP expression by up-regulation of Histone Acetylation through p38MAPK pathway

  • Received:2010-07-19 Revised:2010-10-14 Online:2011-09-05 Published:2011-09-05
  • Contact: Liang LI E-mail:liliang@ccmu.edu.cn

摘要: 摘要:目的 研究p38MAPK信号通路的激活对淀粉前体蛋白(β-Amyloid precursor protein, APP)表达的影响及其相关表观遗传学机制。方法 采用体外培养神经母细胞瘤细胞(SH-SY5Y),Western blot方法检测p38MAPK信号通路激活后APP蛋白的表达及组蛋白乙酰化酶(Histone Acetyltranferase, HAT)和组蛋白去乙酰化酶(Histone Deacetyltranferase, HDAC)的表达情况;光密度值法检测组蛋白H3和H4整体乙酰化水平。结果 p38MAPK信号通路经特异性激动剂激活72小时后,APP表达明显增高至对照组1.5倍;同时组蛋白H3整体乙酰化水平增高,但H4乙酰化水平无明显改变;Western blot结果显示,组蛋白乙酰化酶 CBP表达增高至对照组2.5倍,而组蛋白去乙酰化酶HDAC3表达下降至对照组40%。结论 以上结果提示,p38MAPK信号通路可能通过对组蛋白乙酰化水平的调节影响APP蛋白的表达。

关键词: p38MAPK , 组蛋白乙酰化 , 淀粉前体蛋白 , 老年痴呆

Abstract: ABSTRACT Objective To investigate the role of p38MAPK in the expression of β-Amyloid precursor protein (APP) and it’s epigenetic mechanisms. Methods Cultured SH-SY5Y cells were used to test the expression of APP, histone acetyltranferase (HAT) and histone deacetyltranferase (HDAC) by Western blot after activation of p38MAPK; Acetylation level of histone H3 andH4 were examined by optical density assay. Results APP expression was increased after p38MAPK activation for 72 hours compared to the control. Levels of histone H3 acetylation were increased accompanied with up-regulation of CBP and down-regulation of HDAC3. Conclusion APP Expression could be increased by up-regulation of Histone Acetylation through p38MAPK Pathway.

Key words: p38MAPK, Histone Acetylation, β-amyloid precursor protein, Alzheimer’s disease

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